Quantitative trait loci controlling refractoriness to Plasmodium falciparum in natural Anopheles gambiae mosquitoes from a malaria-endemic region in western Kenya

David M. Menge, Daibin Zhong, Tom Guda, Louis Gouagna, John Githure, John C Beier, Guiyun Yan

Research output: Contribution to journalArticle

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Abstract

Natural anopheline populations exhibit much variation in ability to support malaria parasite development, but the genetic mechanisms underlying this variation are not clear. Previous studies in Mali, West Africa, identified two quantitative trait loci (QTL) in Anopheles gambiae mosquitoes that confer refractoriness (failure of oocyst development in mosquito midguts) to natural Plasmodium falciparum parasites. We hypothesize that new QTL may be involved in mosquito refractoriness to malaria parasites and that the frequency of natural refractoriness genotypes may be higher in the basin region of Lake Victoria, East Africa, where malaria transmission intensity and parasite genetic diversity are among the highest in the world. Using field-derived F2 isofemale families and microsatellite marker genotyping, two loci significantly affecting oocyst density were identified: one on chromosome 2 between markers AG2H135 and AG2H603 and the second on chromosome 3 near marker AG3H93. The first locus was detected in three of the five isofemale families studied and colocalized to the same region as Pen3 and pfin1 described in other studies. The second locus was detected in two of the five isofemale families, and it appears to be a new QTL. QTL on chromosome 2 showed significant additive effects while those on chromosome 3 exhibited significant dominant effects. Identification of P. falciparum-refractoriness QTL in natural An. gambiae mosquitoes is critical to the identification of the genes involved in malaria parasite transmission in nature and for understanding the coevolution between malaria parasites and mosquito vectors.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalGenetics
Volume173
Issue number1
DOIs
StatePublished - May 1 2006

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Anopheles gambiae
Kenya
Quantitative Trait Loci
Plasmodium falciparum
Culicidae
Malaria
Parasites
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 2
Oocysts
Mali
Eastern Africa
Western Africa
Victoria
Lakes
Microsatellite Repeats
Genotype
Population
Genes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Quantitative trait loci controlling refractoriness to Plasmodium falciparum in natural Anopheles gambiae mosquitoes from a malaria-endemic region in western Kenya. / Menge, David M.; Zhong, Daibin; Guda, Tom; Gouagna, Louis; Githure, John; Beier, John C; Yan, Guiyun.

In: Genetics, Vol. 173, No. 1, 01.05.2006, p. 235-241.

Research output: Contribution to journalArticle

Menge, David M. ; Zhong, Daibin ; Guda, Tom ; Gouagna, Louis ; Githure, John ; Beier, John C ; Yan, Guiyun. / Quantitative trait loci controlling refractoriness to Plasmodium falciparum in natural Anopheles gambiae mosquitoes from a malaria-endemic region in western Kenya. In: Genetics. 2006 ; Vol. 173, No. 1. pp. 235-241.
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