Abstract
Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 93-104 |
| Number of pages | 12 |
| Volume | 1322 |
| DOIs | |
| State | Published - 2015 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1322 |
| ISSN (Print) | 10643745 |
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Keywords
- Costal-2
- Drosophila
- Fused
- Hedgehog
- Quantifi cation
- Quantitative immunoblotting
- Smoothened
- Stoichiometry
- Suppressor of Fused
- Ubitus interruptus
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Quantitative immunoblotting of endogenous hedgehog pathway components. / Farzan, Shohreh F.; Robbins, David J.
Methods in Molecular Biology. Vol. 1322 Humana Press Inc., 2015. p. 93-104 (Methods in Molecular Biology; Vol. 1322).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Quantitative immunoblotting of endogenous hedgehog pathway components
AU - Farzan, Shohreh F.
AU - Robbins, David J
PY - 2015
Y1 - 2015
N2 - Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.
AB - Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.
KW - Costal-2
KW - Drosophila
KW - Fused
KW - Hedgehog
KW - Quantifi cation
KW - Quantitative immunoblotting
KW - Smoothened
KW - Stoichiometry
KW - Suppressor of Fused
KW - Ubitus interruptus
UR - http://www.scopus.com/inward/record.url?scp=84946398617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84946398617&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2772-2_9
DO - 10.1007/978-1-4939-2772-2_9
M3 - Chapter
VL - 1322
T3 - Methods in Molecular Biology
SP - 93
EP - 104
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -