Quantitative immunoblotting of endogenous hedgehog pathway components

Shohreh F. Farzan; David J Robbins

doi:10.1007/978-1-4939-2772-2_9

Quantitative immunoblotting of endogenous hedgehog pathway components

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	93-104
Number of pages	12
Volume	1322
DOIs	https://doi.org/10.1007/978-1-4939-2772-2_9
State	Published - 2015
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1322
ISSN (Print)	10643745

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Keywords

Costal-2
Drosophila
Fused
Hedgehog
Quantifi cation
Quantitative immunoblotting
Smoothened
Stoichiometry
Suppressor of Fused
Ubitus interruptus

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Farzan, S. F., & Robbins, D. J. (2015). Quantitative immunoblotting of endogenous hedgehog pathway components. In Methods in Molecular Biology (Vol. 1322, pp. 93-104). (Methods in Molecular Biology; Vol. 1322). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2772-2_9

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Access to Document

10.1007/978-1-4939-2772-2_9