@inbook{2a9298dc463949c3bd1fb68c044738ab,
title = "Quantitative immunoblotting of endogenous hedgehog pathway components",
abstract = "Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.",
keywords = "Costal-2, Drosophila, Fused, Hedgehog, Quantifi cation, Quantitative immunoblotting, Smoothened, Stoichiometry, Suppressor of Fused, Ubitus interruptus",
author = "Farzan, {Shohreh F.} and Robbins, {David J}",
year = "2015",
doi = "10.1007/978-1-4939-2772-2_9",
language = "English (US)",
volume = "1322",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "93--104",
booktitle = "Methods in Molecular Biology",
}