Quantitative immunoblotting of endogenous hedgehog pathway components

Shohreh F. Farzan, David J Robbins

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages93-104
Number of pages12
Volume1322
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1322
ISSN (Print)10643745

Fingerprint

Hedgehogs
Immunoblotting
Drosophila
Cultured Cells
Embryonic Structures

Keywords

  • Costal-2
  • Drosophila
  • Fused
  • Hedgehog
  • Quantifi cation
  • Quantitative immunoblotting
  • Smoothened
  • Stoichiometry
  • Suppressor of Fused
  • Ubitus interruptus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Farzan, S. F., & Robbins, D. J. (2015). Quantitative immunoblotting of endogenous hedgehog pathway components. In Methods in Molecular Biology (Vol. 1322, pp. 93-104). (Methods in Molecular Biology; Vol. 1322). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2772-2_9

Quantitative immunoblotting of endogenous hedgehog pathway components. / Farzan, Shohreh F.; Robbins, David J.

Methods in Molecular Biology. Vol. 1322 Humana Press Inc., 2015. p. 93-104 (Methods in Molecular Biology; Vol. 1322).

Research output: Chapter in Book/Report/Conference proceedingChapter

Farzan, SF & Robbins, DJ 2015, Quantitative immunoblotting of endogenous hedgehog pathway components. in Methods in Molecular Biology. vol. 1322, Methods in Molecular Biology, vol. 1322, Humana Press Inc., pp. 93-104. https://doi.org/10.1007/978-1-4939-2772-2_9
Farzan SF, Robbins DJ. Quantitative immunoblotting of endogenous hedgehog pathway components. In Methods in Molecular Biology. Vol. 1322. Humana Press Inc. 2015. p. 93-104. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2772-2_9
Farzan, Shohreh F. ; Robbins, David J. / Quantitative immunoblotting of endogenous hedgehog pathway components. Methods in Molecular Biology. Vol. 1322 Humana Press Inc., 2015. pp. 93-104 (Methods in Molecular Biology).
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