Abstract
Quantitative analysis and modeling of signaling pathway components can reveal important information about the dynamics of that system, including the relative stoichiometries and affi nities between the individual signaling components, as well as rate-limiting steps in the signaling pathway. In this chapter, we present a method that we developed to quantify the steady-state ratio of core Hedgehog (Hh) signaling components in both cultured cells and the Drosophila embryo, a physiologically relevant tissue.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 93-104 |
| Number of pages | 12 |
| Volume | 1322 |
| DOIs | |
| State | Published - 2015 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1322 |
| ISSN (Print) | 10643745 |
Keywords
- Costal-2
- Drosophila
- Fused
- Hedgehog
- Quantifi cation
- Quantitative immunoblotting
- Smoothened
- Stoichiometry
- Suppressor of Fused
- Ubitus interruptus
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Farzan, S. F., & Robbins, D. J. (2015). Quantitative immunoblotting of endogenous hedgehog pathway components. In Methods in Molecular Biology (Vol. 1322, pp. 93-104). (Methods in Molecular Biology; Vol. 1322). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2772-2_9