Abstract
An ultra high resolution spectral-domain optical coherence tomography (SD-OCT) together with an advanced animal restraint and positioning system was built for noninvasive non-contact in vivo three-dimensional imaging of rodent models of ocular diseases. The animal positioning system allowed the operator to rapidly locate and switch the areas of interest on the retina. This function together with the capability of precise spatial registration provided by the generated OCT fundus image allows the system to locate and compare the same lesion (retinal tumor in the current study) at different time point throughout the entire course of the disease progression. An algorithm for fully automatic segmentation of the tumor boundaries and calculation of tumor volume was developed. The system and algorithm were successfully applied to monitoring retinal tumor growth quantitatively over time in the LHBETAT AG mouse model of retinoblastoma.
Original language | English (US) |
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Pages (from-to) | 17-28 |
Number of pages | 12 |
Journal | Journal of Innovative Optical Health Sciences |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Jun 2008 |
Keywords
- 3D imaging
- image segmentation
- mouse models
- optical coherence tomography
- retina
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Medicine (miscellaneous)
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering