Quantitative differential expression analysis reveals miR-7 as major islet microRNA

Valia Bravo-Egana, Samuel Rosero, Ruth Molano, Antonello Pileggi, Camillo Ricordi, Juan Dominguez-Bendala, Ricardo Pastori

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

Original languageEnglish
Pages (from-to)922-926
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number4
DOIs
StatePublished - Feb 22 2008

Fingerprint

MicroRNAs
Gene expression
Gene Expression
Microarrays
Islets of Langerhans
Genes
Maintenance
Technology
Phenotype
Polymerase Chain Reaction
Messenger RNA

Keywords

  • MicroRNA microarrays
  • MicroRNAs
  • Pancreatic islets
  • q-RT-PCR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quantitative differential expression analysis reveals miR-7 as major islet microRNA. / Bravo-Egana, Valia; Rosero, Samuel; Molano, Ruth; Pileggi, Antonello; Ricordi, Camillo; Dominguez-Bendala, Juan; Pastori, Ricardo.

In: Biochemical and Biophysical Research Communications, Vol. 366, No. 4, 22.02.2008, p. 922-926.

Research output: Contribution to journalArticle

@article{d4e6f71f4a34473d95261e35aaa49c6e,
title = "Quantitative differential expression analysis reveals miR-7 as major islet microRNA",
abstract = "MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.",
keywords = "MicroRNA microarrays, MicroRNAs, Pancreatic islets, q-RT-PCR",
author = "Valia Bravo-Egana and Samuel Rosero and Ruth Molano and Antonello Pileggi and Camillo Ricordi and Juan Dominguez-Bendala and Ricardo Pastori",
year = "2008",
month = "2",
day = "22",
doi = "10.1016/j.bbrc.2007.12.052",
language = "English",
volume = "366",
pages = "922--926",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Quantitative differential expression analysis reveals miR-7 as major islet microRNA

AU - Bravo-Egana, Valia

AU - Rosero, Samuel

AU - Molano, Ruth

AU - Pileggi, Antonello

AU - Ricordi, Camillo

AU - Dominguez-Bendala, Juan

AU - Pastori, Ricardo

PY - 2008/2/22

Y1 - 2008/2/22

N2 - MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

AB - MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

KW - MicroRNA microarrays

KW - MicroRNAs

KW - Pancreatic islets

KW - q-RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=37549021902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37549021902&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.12.052

DO - 10.1016/j.bbrc.2007.12.052

M3 - Article

C2 - 18086561

AN - SCOPUS:37549021902

VL - 366

SP - 922

EP - 926

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -