TY - JOUR
T1 - Quantitative determination of bioactive constituents in Noni Juice by High-performance liquid chromatography with electrospray ionization triple quadrupole mass spectrometry
AU - Yan, Yongqiu
AU - Lu, Yu
AU - Jiang, Shiping
AU - Jiang, Yu
AU - Tong, Yingpeng
AU - Zuo, Limin
AU - Yang, Jun
AU - Gong, Feng
AU - Zhang, Ling
AU - Wang, Ping
N1 - Funding Information:
Major Scientific and Technological Special Projects for Key Agricultural Program of Zhejiang Province (No. 2015C02032); China Postdoctoral Science Foundation (No. 2016M592016) and Special Project of International Technology Cooperation of One Belt and One Road (No. 2017C04010).
Publisher Copyright:
© 2018 Pharmacognosy Magazine Published by Wolters Kluwer-Medknow.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Background: Noni juice has been extensively used as folk medicine for the treatment of arthritis, infections, analgesic, colds, cancers, and diabetes by Polynesians for many years. Due to the lack of standard scientific evaluation methods, various kinds of commercial Noni juice with different quality and price were available on the market. Objective: To establish a sensitive, reliable, and accurate high-performance liquid chromatography with electrospray ionization triple quadrupole mass spectrometry (HPLC-ESI-MS/MS) method for separation, identification, and simultaneous quantitative analysis of bioactive constituents in Noni juice. Materials and Methods: The analytes and eight batches of commercially available samples from different origins were separated and analyzed by the HPLC-ESI-MS/MS method on an Agilent ZORBAX SB-C18(150 mm × 4.6 mm i.d., 5 μm) column using a gradient elution of acetonitrile-methanol-0.05% glacial acetic acid in water (v/v) at a constant flow rate of 0.5 mL/min. Results: Seven components were identification and all of the assay parameters were within the required limits. Components were within the correlation coefficient values (R2 ≥ 0.9993) at the concentration ranges tested. The precision of the assay method was <0.91% and the repeatability between 1.36% and 3.31%. The accuracy varied from 96.40% to 103.02% and the relative standard deviations of stability were <3.91%. Samples from the same origin showed similar content while different origins showed significant different result. Conclusions: The developed methods would provide a reliable basis and be useful in the establishment of a rational quality control standard of Noni juice.
AB - Background: Noni juice has been extensively used as folk medicine for the treatment of arthritis, infections, analgesic, colds, cancers, and diabetes by Polynesians for many years. Due to the lack of standard scientific evaluation methods, various kinds of commercial Noni juice with different quality and price were available on the market. Objective: To establish a sensitive, reliable, and accurate high-performance liquid chromatography with electrospray ionization triple quadrupole mass spectrometry (HPLC-ESI-MS/MS) method for separation, identification, and simultaneous quantitative analysis of bioactive constituents in Noni juice. Materials and Methods: The analytes and eight batches of commercially available samples from different origins were separated and analyzed by the HPLC-ESI-MS/MS method on an Agilent ZORBAX SB-C18(150 mm × 4.6 mm i.d., 5 μm) column using a gradient elution of acetonitrile-methanol-0.05% glacial acetic acid in water (v/v) at a constant flow rate of 0.5 mL/min. Results: Seven components were identification and all of the assay parameters were within the required limits. Components were within the correlation coefficient values (R2 ≥ 0.9993) at the concentration ranges tested. The precision of the assay method was <0.91% and the repeatability between 1.36% and 3.31%. The accuracy varied from 96.40% to 103.02% and the relative standard deviations of stability were <3.91%. Samples from the same origin showed similar content while different origins showed significant different result. Conclusions: The developed methods would provide a reliable basis and be useful in the establishment of a rational quality control standard of Noni juice.
KW - Bioactive constituents
KW - Noni juice
KW - high-performance liquid chromatography with electrospray ionization triple quadrupole mass spectrometry
KW - quantitative determination
UR - http://www.scopus.com/inward/record.url?scp=85042550724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042550724&partnerID=8YFLogxK
U2 - 10.4103/pm.pm_74_17
DO - 10.4103/pm.pm_74_17
M3 - Article
AN - SCOPUS:85042550724
VL - 14
SP - 70
EP - 75
JO - Pharmacognosy Magazine
JF - Pharmacognosy Magazine
SN - 0973-1296
IS - 53
ER -