Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis

Maria De Medina, Silvia LaRue, Mary Hill, Howard O'Sullivan, J. Phillip Pennell, B. Leclercq, Xiuming Li, Lennox J Jeffers, Talley Parker, K. Rajender Reddy, Eugene R Schiff, Guido O. Perez

Research output: Contribution to journalArticle

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Abstract

The purpose of the current study was to detect quantitatively hepatitis C virus (HCV)-RNA among patients undergoing maintenance hemodialysis. Study subjects were 88 patients on hemodialysis at the Miami Veterans Administration Medical Center and the REN Dialysis Unit at the University of Miami School of Medicine. There were 66 men and 22 women, mean age 52 years (range, 22-87 years), and mean duration of dialysis was 2.8 years (range, 0.2-12.5 years). Seventy-three percent had a history of blood transfusion. Anti-HCV was determined by enzyme linked immunosorbent assay (ELISA), confirmed by four antigen strip immunoblot assay (RIBA 2.0 SIA). HCV-RNA was quantitated directly in human sera using a branched DNA (bDNA) signal amplification assay. Twenty-seven of 88 (31%) patient samples were found to be anti-HCV reactive by ELISA. Twenty-two of 27 were confirmed reactive, 2 were indeterminate, and 3 were nonreactive by RIBA HCV. Eighteen of 22 (82%) reactive by RIBA 2.0 HVC were found to have detectable (>3.5 x 105 Eq/ml) HCV-RNA levels (mean [± SD], 43.3 ± 35.4 x 105 Eq/ml; range, 4.9-123.2). No additional cases were identified with reverse transcription polymerase chain reaction (RT-PCR) using 5' untranslated region 'nested' primers. HCV- RNA was not detected in four RIBA HCV 2.0 reactive, the two indeterminate, or the 64 patient samples nonreactive for anti-HCV. The two epitopes most commonly associated with HCV-RNA were c22-3 and c33c. Sixteen of 18 (89%) patients with detectable levels of HCV-RNA had normal alanine aminotransferase (ALT). Three patients with the highest levels of HCV-RNA were infected with the human immunodeficiency virus. The authors conclude that HCV-RNA by bDNA assay is a sensitive, specific, and simple test that can be used in association with antibody assays and a PCR-based assay to study the prevalence and management of HCV infection in the dialysis setting.

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalASAIO Journal
Volume43
Issue number1
DOIs
StatePublished - Feb 1 1997

Fingerprint

RNA
Viruses
Hepacivirus
Renal Dialysis
Assays
Dialysis
Branched DNA Signal Amplification Assay
Immunosorbents
Enzyme-Linked Immunosorbent Assay
DNA
Enzymes
Polymerase Chain Reaction
United States Department of Veterans Affairs
Epitopes
5' Untranslated Regions
Virus Diseases
Polymerase chain reaction
Alanine Transaminase
Transcription
Antigens

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

De Medina, M., LaRue, S., Hill, M., O'Sullivan, H., Pennell, J. P., Leclercq, B., ... Perez, G. O. (1997). Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis. ASAIO Journal, 43(1), 19-22. https://doi.org/10.1097/00002480-199701000-00005

Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis. / De Medina, Maria; LaRue, Silvia; Hill, Mary; O'Sullivan, Howard; Pennell, J. Phillip; Leclercq, B.; Li, Xiuming; Jeffers, Lennox J; Parker, Talley; Reddy, K. Rajender; Schiff, Eugene R; Perez, Guido O.

In: ASAIO Journal, Vol. 43, No. 1, 01.02.1997, p. 19-22.

Research output: Contribution to journalArticle

De Medina, M, LaRue, S, Hill, M, O'Sullivan, H, Pennell, JP, Leclercq, B, Li, X, Jeffers, LJ, Parker, T, Reddy, KR, Schiff, ER & Perez, GO 1997, 'Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis', ASAIO Journal, vol. 43, no. 1, pp. 19-22. https://doi.org/10.1097/00002480-199701000-00005
De Medina M, LaRue S, Hill M, O'Sullivan H, Pennell JP, Leclercq B et al. Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis. ASAIO Journal. 1997 Feb 1;43(1):19-22. https://doi.org/10.1097/00002480-199701000-00005
De Medina, Maria ; LaRue, Silvia ; Hill, Mary ; O'Sullivan, Howard ; Pennell, J. Phillip ; Leclercq, B. ; Li, Xiuming ; Jeffers, Lennox J ; Parker, Talley ; Reddy, K. Rajender ; Schiff, Eugene R ; Perez, Guido O. / Quantitative detection of hepatitis C virus RNA in patients undergoing hemodialysis. In: ASAIO Journal. 1997 ; Vol. 43, No. 1. pp. 19-22.
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abstract = "The purpose of the current study was to detect quantitatively hepatitis C virus (HCV)-RNA among patients undergoing maintenance hemodialysis. Study subjects were 88 patients on hemodialysis at the Miami Veterans Administration Medical Center and the REN Dialysis Unit at the University of Miami School of Medicine. There were 66 men and 22 women, mean age 52 years (range, 22-87 years), and mean duration of dialysis was 2.8 years (range, 0.2-12.5 years). Seventy-three percent had a history of blood transfusion. Anti-HCV was determined by enzyme linked immunosorbent assay (ELISA), confirmed by four antigen strip immunoblot assay (RIBA 2.0 SIA). HCV-RNA was quantitated directly in human sera using a branched DNA (bDNA) signal amplification assay. Twenty-seven of 88 (31{\%}) patient samples were found to be anti-HCV reactive by ELISA. Twenty-two of 27 were confirmed reactive, 2 were indeterminate, and 3 were nonreactive by RIBA HCV. Eighteen of 22 (82{\%}) reactive by RIBA 2.0 HVC were found to have detectable (>3.5 x 105 Eq/ml) HCV-RNA levels (mean [± SD], 43.3 ± 35.4 x 105 Eq/ml; range, 4.9-123.2). No additional cases were identified with reverse transcription polymerase chain reaction (RT-PCR) using 5' untranslated region 'nested' primers. HCV- RNA was not detected in four RIBA HCV 2.0 reactive, the two indeterminate, or the 64 patient samples nonreactive for anti-HCV. The two epitopes most commonly associated with HCV-RNA were c22-3 and c33c. Sixteen of 18 (89{\%}) patients with detectable levels of HCV-RNA had normal alanine aminotransferase (ALT). Three patients with the highest levels of HCV-RNA were infected with the human immunodeficiency virus. The authors conclude that HCV-RNA by bDNA assay is a sensitive, specific, and simple test that can be used in association with antibody assays and a PCR-based assay to study the prevalence and management of HCV infection in the dialysis setting.",
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