Quantitative description of three modes of activity of fast chloride channels from rat skeletal muscle

A. L. Blatz, Karl Magleby

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

The steady-state kinetic properties of single Cl- channels with fast kinetics active at resting membrane potentials in cultured rat skeletal muscle were studied using the patch-clamp technique. Membrane patches containing single active Cl- channels were often observed, and binomial analysis of the percentage open time in membrane patches containing several Cl- channels indicated that the channels did not occur as obligatory dimers and that they gated independently of one another. Channel activity could be divided into three categories: normal, which included about 99% of the openings and closings; buzz mode, which included about 1% and consisted of bursts of about 50 brief open and shut intervals; and inactivated shut states which included about 0.01% of the shut intervals and lasted for seconds, and occasionally minutes. The method of maximum likelihood was used to determine the number of significant exponential components required to fit the distributions of open and shut intervals during normal activity. Open interval distributions required at least two components, with time constants of 0.52 and 1.5 ms at -40 mV and 7.6°C. Shut interval distributions required at least five exponential components, with time constants of 0.064, 0.72, 1.9, 12.3 and 350 ms. Kinetic reaction schemes were developed for the normal and buzz mode using maximum likelihood techniques to determine the most likely models and rate constants. In developing these models the effects of limited time resolution and missed events were taken into account. Each model tested typically had two or more sets of equally likely rate constants. Incorrect sets of rate constants resulting from the effect of missed events could be eliminated by analysis of the data with different time resolutions. Normal activity could be accounted for by several different seven-state models with two open and five shut states. As different models could be found that gave identical descriptions of the data, the distributions of open and shut intervals were not sufficient to define a unique model. It was established that no other seven-state models would be found that describe the distributions of open and shut intervals during normal activity better than the most likely presented models. Amplitude histograms of single-channel current during normal activity contained a single open peak, suggesting that the two open-channel states are of similar conductance. During activity in the buzz mode the distributions of open and shut intervals were approximated by single exponentials, with time constants of 0.096 and 0.23 ms, respectively. A two-state model with mean open and shut lifetimes of 0.058 and 0.103 ms, respectively, could account for the activity. Increased filtering over that used in the analysis made activity in the buzz mode appear as a partially conducting state because of the time averaging of current during the brief open and shut lifetimes. These results indicate that the Cl- channel has ten or more states. The channel enters at least two open and five shut states during normal activity. An additional open and shut state are entered during the buzz mode, and one or more additional shut states are entered during the inactivated periods. A number of the kinetic properties of this anion channel are similar to those described for cation channels such as the acetylcholine receptor channel and a large conductance Ca2+-activated K+ channel.

Original languageEnglish
Pages (from-to)141-174
Number of pages34
JournalJournal of Physiology
VolumeVol. 378
StatePublished - Jan 1 1986
Externally publishedYes

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Chloride Channels
Skeletal Muscle
Calcium-Activated Potassium Channels
Membranes
Cholinergic Receptors
Patch-Clamp Techniques
Membrane Potentials
Anions
Cations

ASJC Scopus subject areas

  • Physiology

Cite this

Quantitative description of three modes of activity of fast chloride channels from rat skeletal muscle. / Blatz, A. L.; Magleby, Karl.

In: Journal of Physiology, Vol. Vol. 378, 01.01.1986, p. 141-174.

Research output: Contribution to journalArticle

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N2 - The steady-state kinetic properties of single Cl- channels with fast kinetics active at resting membrane potentials in cultured rat skeletal muscle were studied using the patch-clamp technique. Membrane patches containing single active Cl- channels were often observed, and binomial analysis of the percentage open time in membrane patches containing several Cl- channels indicated that the channels did not occur as obligatory dimers and that they gated independently of one another. Channel activity could be divided into three categories: normal, which included about 99% of the openings and closings; buzz mode, which included about 1% and consisted of bursts of about 50 brief open and shut intervals; and inactivated shut states which included about 0.01% of the shut intervals and lasted for seconds, and occasionally minutes. The method of maximum likelihood was used to determine the number of significant exponential components required to fit the distributions of open and shut intervals during normal activity. Open interval distributions required at least two components, with time constants of 0.52 and 1.5 ms at -40 mV and 7.6°C. Shut interval distributions required at least five exponential components, with time constants of 0.064, 0.72, 1.9, 12.3 and 350 ms. Kinetic reaction schemes were developed for the normal and buzz mode using maximum likelihood techniques to determine the most likely models and rate constants. In developing these models the effects of limited time resolution and missed events were taken into account. Each model tested typically had two or more sets of equally likely rate constants. Incorrect sets of rate constants resulting from the effect of missed events could be eliminated by analysis of the data with different time resolutions. Normal activity could be accounted for by several different seven-state models with two open and five shut states. As different models could be found that gave identical descriptions of the data, the distributions of open and shut intervals were not sufficient to define a unique model. It was established that no other seven-state models would be found that describe the distributions of open and shut intervals during normal activity better than the most likely presented models. Amplitude histograms of single-channel current during normal activity contained a single open peak, suggesting that the two open-channel states are of similar conductance. During activity in the buzz mode the distributions of open and shut intervals were approximated by single exponentials, with time constants of 0.096 and 0.23 ms, respectively. A two-state model with mean open and shut lifetimes of 0.058 and 0.103 ms, respectively, could account for the activity. Increased filtering over that used in the analysis made activity in the buzz mode appear as a partially conducting state because of the time averaging of current during the brief open and shut lifetimes. These results indicate that the Cl- channel has ten or more states. The channel enters at least two open and five shut states during normal activity. An additional open and shut state are entered during the buzz mode, and one or more additional shut states are entered during the inactivated periods. A number of the kinetic properties of this anion channel are similar to those described for cation channels such as the acetylcholine receptor channel and a large conductance Ca2+-activated K+ channel.

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