Quantitative competitive RNA PCR for quantitation of virion-associated HIV-1 RNA

Beda Brichacek, Mario Stevenson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Quantitative competitive PCR is a highly sensitive technique that allows accurate quantitation of small amounts of RNA. We have modified the original method to include the use of an internal standard at all stages of sample analysis. In this way, the method can accommodate for variations in the recovery of viral particles and in the isolation of genomic RNA as well as provide a suitable competitive substrate during quantitative RNA PCR. We have used this method to characterize changes in virus load in plasma of HIV-1-seropositive individuals following their vaccination against opportunistic infections.

Original languageEnglish
Pages (from-to)294-299
Number of pages6
JournalMethods: A Companion to Methods in Enzymology
Volume12
Issue number4
DOIs
StatePublished - Aug 1 1997
Externally publishedYes

Fingerprint

Virion
HIV-1
RNA
Polymerase Chain Reaction
Opportunistic Infections
Viruses
Vaccination
Plasmas
Recovery
Substrates

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Quantitative competitive RNA PCR for quantitation of virion-associated HIV-1 RNA. / Brichacek, Beda; Stevenson, Mario.

In: Methods: A Companion to Methods in Enzymology, Vol. 12, No. 4, 01.08.1997, p. 294-299.

Research output: Contribution to journalArticle

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