Quantitative codon optimisation of DNA libraries encoding sub-random peptides

design and characterisation of a novel library encoding transmembrane domain peptides.

Ola Larsson, Dorit Thormeyer, Arian Asinger, Björn Wihlén, Claes R Wahlestedt, Zicai Liang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Codons for amino acids sharing similar chemical properties seem to cluster on the genetic codon table. Such a geographical distribution of the codons was exploited to create chemically synthesised DNA that encodes peptide libraries containing only a subset of the 20 natural amino acids. The frequency of each amino acid in the subset was further optimised by quantitatively manipulating the ratio of the four phosphoamidites during chemical synthesis of the libraries. Peptides encoded by such libraries show a reduced complexity and could be enriched in peptides of a desired property, which are thus more suitable when screening for functional peptides. Proof of concept for the codon-biased design of peptide libraries was shown by design, synthesis, and characterisation of a transmembrane peptide library that contains >80% transmembrane peptides, representing a 160-fold enrichment compared with a fully randomised library.

Original languageEnglish
JournalNucleic Acids Research
Volume30
Issue number23
StatePublished - Dec 1 2002
Externally publishedYes

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Gene Library
Peptide Library
Codon
Libraries
Peptides
Amino Acids
Small Molecule Libraries
Protein Domains
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Quantitative codon optimisation of DNA libraries encoding sub-random peptides : design and characterisation of a novel library encoding transmembrane domain peptides. / Larsson, Ola; Thormeyer, Dorit; Asinger, Arian; Wihlén, Björn; Wahlestedt, Claes R; Liang, Zicai.

In: Nucleic Acids Research, Vol. 30, No. 23, 01.12.2002.

Research output: Contribution to journalArticle

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