Quantitative assessment of HCV load in chronic hemodialysis patients: A cross-sectional survey

F. Fabrizi, Paul Martin, V. Dixit, M. Brezina, M. J. Cole, S. Gerosa, S. Vinson, M. Mousa, G. Gitnick

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Recent evidence has been accumulated showing that chronic hemodialysis (HD) patients have a very high prevalence of antibodies to hepatitis C virus (HCV). In contrast, there is little information addressing the virological characteristics of HCV infection in this population. Aim: To measure HCV viral load and to correlate this with demographic, biochemical, and clinical features of a large cohort of HCV-infected patients on chronic HD. Methods: 394 chronic HD patients were tested by branched-DNA signal amplification assay, anti-HCV enzyme-linked immunosorbent assay 2.0, and on the basis of the aspartate aminotransferase/alanine aminotransferase (AST/ALT) activity. Multivariate analysis by ordinal logistic regression model was performed: age, gender, race, time on HD, allocation of the patients among the HD units, etiology of end-stage renal disease, HBsAg status, anti-HCV positivity, HCV genotype, and AST/ALT levels were independent factors, and viremic levels of HCV in serum were assumed as dependent variables. Results: 88 (22.3%) patients showed serological and/or virological signs of HCV infection, 59 (15%) out of 394 had detectable HCV RNA in serum, the mean HCV load was 19.4 x 105 (95% CI, 6.06 x 107 to 6.2 x 104) Eq/ml. According to the criteria suggested by others, there were 8 (13.5%) individuals with high-titer viremia (> 1 x 107 Eq/ml) in the subset of viremic patients, A small subset (8/394 or 2%) of individuals was seronegative, but viremic; 29 (7%) out of 394 were seropositive without detectable HCV RNA in serum. Univariate analysis showed that the frequency of anti-HCV positivity was significantly higher in viremic patients as compared with individuals with no detectable HCV viremia: 51/59 (86%) vs, 29/335 (8.6%), p = 0.0001, Serum AST and ALT levels were significantly higher in viremic patients than in individuals with no detectable HCV RNA in serum: 23.8 (95% CI 60, 8-9.3) vs. 17.1 (95% CI 50.4-5.8) U/1 (p = 0.009) and 14.4 (95% CI 48.9-4.3) vs, 9.8 (95% CI, 37.3-2.5) U/1 (p = 0.009), Logistic regression analysis showed an association between HCV viremia and anti-HCV positivity (p = 0.00001) and ALT activity (p = 0.01), Conclusions: Hepatitis C virus infection is highly prevalent in the HD population; the viral load is relatively low, and it was associated with elevated hepatic enzyme levels and anti-HCV positivity, No other clinical characteristics were associated with HCV RNA levels. Seronegative but viremic patients were also found, Longitudinal studies with long follow-up periods are necessary to evaluate the course of HCV load overtime in this population.

Original languageEnglish
Pages (from-to)428-433
Number of pages6
JournalNephron
Volume80
Issue number4
StatePublished - Dec 17 1998
Externally publishedYes

Fingerprint

Hepacivirus
Renal Dialysis
Cross-Sectional Studies
Viremia
Virus Diseases
RNA
Serum
Logistic Models
Aspartate Aminotransferases
Viral Load
Alanine Transaminase
Branched DNA Signal Amplification Assay
Population
Hepatitis C Antibodies
Hepatitis B Surface Antigens

Keywords

  • Hemodialysis, viremia
  • Hepatitis C virus infection
  • Viral load, hepatitis C

ASJC Scopus subject areas

  • Nephrology

Cite this

Fabrizi, F., Martin, P., Dixit, V., Brezina, M., Cole, M. J., Gerosa, S., ... Gitnick, G. (1998). Quantitative assessment of HCV load in chronic hemodialysis patients: A cross-sectional survey. Nephron, 80(4), 428-433.

Quantitative assessment of HCV load in chronic hemodialysis patients : A cross-sectional survey. / Fabrizi, F.; Martin, Paul; Dixit, V.; Brezina, M.; Cole, M. J.; Gerosa, S.; Vinson, S.; Mousa, M.; Gitnick, G.

In: Nephron, Vol. 80, No. 4, 17.12.1998, p. 428-433.

Research output: Contribution to journalArticle

Fabrizi, F, Martin, P, Dixit, V, Brezina, M, Cole, MJ, Gerosa, S, Vinson, S, Mousa, M & Gitnick, G 1998, 'Quantitative assessment of HCV load in chronic hemodialysis patients: A cross-sectional survey', Nephron, vol. 80, no. 4, pp. 428-433.
Fabrizi F, Martin P, Dixit V, Brezina M, Cole MJ, Gerosa S et al. Quantitative assessment of HCV load in chronic hemodialysis patients: A cross-sectional survey. Nephron. 1998 Dec 17;80(4):428-433.
Fabrizi, F. ; Martin, Paul ; Dixit, V. ; Brezina, M. ; Cole, M. J. ; Gerosa, S. ; Vinson, S. ; Mousa, M. ; Gitnick, G. / Quantitative assessment of HCV load in chronic hemodialysis patients : A cross-sectional survey. In: Nephron. 1998 ; Vol. 80, No. 4. pp. 428-433.
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T1 - Quantitative assessment of HCV load in chronic hemodialysis patients

T2 - A cross-sectional survey

AU - Fabrizi, F.

AU - Martin, Paul

AU - Dixit, V.

AU - Brezina, M.

AU - Cole, M. J.

AU - Gerosa, S.

AU - Vinson, S.

AU - Mousa, M.

AU - Gitnick, G.

PY - 1998/12/17

Y1 - 1998/12/17

N2 - Recent evidence has been accumulated showing that chronic hemodialysis (HD) patients have a very high prevalence of antibodies to hepatitis C virus (HCV). In contrast, there is little information addressing the virological characteristics of HCV infection in this population. Aim: To measure HCV viral load and to correlate this with demographic, biochemical, and clinical features of a large cohort of HCV-infected patients on chronic HD. Methods: 394 chronic HD patients were tested by branched-DNA signal amplification assay, anti-HCV enzyme-linked immunosorbent assay 2.0, and on the basis of the aspartate aminotransferase/alanine aminotransferase (AST/ALT) activity. Multivariate analysis by ordinal logistic regression model was performed: age, gender, race, time on HD, allocation of the patients among the HD units, etiology of end-stage renal disease, HBsAg status, anti-HCV positivity, HCV genotype, and AST/ALT levels were independent factors, and viremic levels of HCV in serum were assumed as dependent variables. Results: 88 (22.3%) patients showed serological and/or virological signs of HCV infection, 59 (15%) out of 394 had detectable HCV RNA in serum, the mean HCV load was 19.4 x 105 (95% CI, 6.06 x 107 to 6.2 x 104) Eq/ml. According to the criteria suggested by others, there were 8 (13.5%) individuals with high-titer viremia (> 1 x 107 Eq/ml) in the subset of viremic patients, A small subset (8/394 or 2%) of individuals was seronegative, but viremic; 29 (7%) out of 394 were seropositive without detectable HCV RNA in serum. Univariate analysis showed that the frequency of anti-HCV positivity was significantly higher in viremic patients as compared with individuals with no detectable HCV viremia: 51/59 (86%) vs, 29/335 (8.6%), p = 0.0001, Serum AST and ALT levels were significantly higher in viremic patients than in individuals with no detectable HCV RNA in serum: 23.8 (95% CI 60, 8-9.3) vs. 17.1 (95% CI 50.4-5.8) U/1 (p = 0.009) and 14.4 (95% CI 48.9-4.3) vs, 9.8 (95% CI, 37.3-2.5) U/1 (p = 0.009), Logistic regression analysis showed an association between HCV viremia and anti-HCV positivity (p = 0.00001) and ALT activity (p = 0.01), Conclusions: Hepatitis C virus infection is highly prevalent in the HD population; the viral load is relatively low, and it was associated with elevated hepatic enzyme levels and anti-HCV positivity, No other clinical characteristics were associated with HCV RNA levels. Seronegative but viremic patients were also found, Longitudinal studies with long follow-up periods are necessary to evaluate the course of HCV load overtime in this population.

AB - Recent evidence has been accumulated showing that chronic hemodialysis (HD) patients have a very high prevalence of antibodies to hepatitis C virus (HCV). In contrast, there is little information addressing the virological characteristics of HCV infection in this population. Aim: To measure HCV viral load and to correlate this with demographic, biochemical, and clinical features of a large cohort of HCV-infected patients on chronic HD. Methods: 394 chronic HD patients were tested by branched-DNA signal amplification assay, anti-HCV enzyme-linked immunosorbent assay 2.0, and on the basis of the aspartate aminotransferase/alanine aminotransferase (AST/ALT) activity. Multivariate analysis by ordinal logistic regression model was performed: age, gender, race, time on HD, allocation of the patients among the HD units, etiology of end-stage renal disease, HBsAg status, anti-HCV positivity, HCV genotype, and AST/ALT levels were independent factors, and viremic levels of HCV in serum were assumed as dependent variables. Results: 88 (22.3%) patients showed serological and/or virological signs of HCV infection, 59 (15%) out of 394 had detectable HCV RNA in serum, the mean HCV load was 19.4 x 105 (95% CI, 6.06 x 107 to 6.2 x 104) Eq/ml. According to the criteria suggested by others, there were 8 (13.5%) individuals with high-titer viremia (> 1 x 107 Eq/ml) in the subset of viremic patients, A small subset (8/394 or 2%) of individuals was seronegative, but viremic; 29 (7%) out of 394 were seropositive without detectable HCV RNA in serum. Univariate analysis showed that the frequency of anti-HCV positivity was significantly higher in viremic patients as compared with individuals with no detectable HCV viremia: 51/59 (86%) vs, 29/335 (8.6%), p = 0.0001, Serum AST and ALT levels were significantly higher in viremic patients than in individuals with no detectable HCV RNA in serum: 23.8 (95% CI 60, 8-9.3) vs. 17.1 (95% CI 50.4-5.8) U/1 (p = 0.009) and 14.4 (95% CI 48.9-4.3) vs, 9.8 (95% CI, 37.3-2.5) U/1 (p = 0.009), Logistic regression analysis showed an association between HCV viremia and anti-HCV positivity (p = 0.00001) and ALT activity (p = 0.01), Conclusions: Hepatitis C virus infection is highly prevalent in the HD population; the viral load is relatively low, and it was associated with elevated hepatic enzyme levels and anti-HCV positivity, No other clinical characteristics were associated with HCV RNA levels. Seronegative but viremic patients were also found, Longitudinal studies with long follow-up periods are necessary to evaluate the course of HCV load overtime in this population.

KW - Hemodialysis, viremia

KW - Hepatitis C virus infection

KW - Viral load, hepatitis C

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