Quantitative analysis of the intraretinal layers and optic nerve head using ultra-high resolution optical coherence tomography

Yuhong Wang, Hong Jiang, Meixiao Shen, Byron L Lam, Delia Cabrera DeBuc, Yufeng Ye, Ming Li, Aizhu Tao, Yilei Shao, Jianhua Wang

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12 Scopus citations


This study is designed to test the repeatability of the quantitative analysis of intraretinal layer thickness and cup-disc ratio of the optic nerve head using ultra-high resolution optical coherence tomography (UHR-OCT). Group A, containing 23 eyes of 12 healthy subjects, was imaged twice and group B, containing eight eyes of four subjects, was imaged three times. Intraretinal layers were segmented manually and the cup-to-disc ratio of the optic nerve head was analyzed. Custom-built automatic segmentation software was also used to segment a set of images for comparison. A total of nine intraretinal layers were visualized and extracted manually. With group A, the central foveal thickness was 186.4 15.9 ?m (mean SD). The average retinal thickness was 296.4 21.3 ?m. The best repeatability, obtained when two repeated scans were taken, was obtained for the outer nuclear layer followed by the ganglion cell layer, the inner nuclear layer, the retinal nerve fiber layer and the worst was obtained for the outer segment. The intraclass correlation ranged from 0.824 to 0.997. The coefficients of repeatability ranged from 3.24 to 18.3 ?m, corresponding to 1.47% to 26.20%. With group B, high interclass correlations were found and the automatic segmentation results were compatible with the manual results. Our results indicated that more retinal features might be imageable using UHR-OCT.

Original languageEnglish
Article number066013
JournalJournal of Biomedical Optics
Issue number6
StatePublished - Jun 1 2012



  • Optic nerve head
  • Quantitative analysis
  • Retinal segment
  • Segmentation image processing
  • Spectral domain
  • Ultra-high resolution optical coherence tomography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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