Quantitative analysis of parasympathetic innervation of the porcine heart

Joseph S. Ulphani, Jack H. Cain, Firdous Inderyas, David Gordon, Peter V. Gikas, Gregory Shade, David Mayor, Rishi Arora, Alan H. Kadish, Jeffrey Goldberger

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background: Parasympathetic control of the heart is an important component in the regulation of normal cardiac function. However, the anatomic course of parasympathetic innervation of the heart is unclear. Objective: The purpose of this study was to apply a gross parasympathetic nerve stain technique to reveal the details of the morphology of the cardiac parasympathetic nervous system. Methods: Ten whole pig hearts were stained using a histochemical method. Immediately after sacrifice, hearts were placed in a buffered solution containing acetylthiocholine, which precipitates with acetylcholinesterase, allowing identification of cholinergic nerves. The epicardial and endocardial surfaces of the atria and ventricles were examined for nerve thickness and density. Results: In both atria, nerve density was significantly greater on the endocardium, but nerve thickness was significantly greater on the epicardium. The right atrium (RA) was more densely innervated than the left atrium (LA) on the endocardium, whereas the LA was more densely innervated than the RA on the epicardium. In the ventricles, numerous thick cholinergic nerves were clearly identifiable across the epicardium, generally running parallel to the left anterior descending artery. The endocardial surfaces of the ventricles revealed a dense network of fine parasympathetic nerve fibers. As in the atria, nerve density was greater on the ventricular endocardium, but nerve thickness was greater on the epicardium. The right ventricle (RV) was more densely innervated than the left ventricle (LV), whereas the LV endocardium was more densely innervated than the RV endocardium. Conclusion: The epicardial and endocardial surfaces of the atria and ventricles are richly innervated by parasympathetic nerves. The density of parasympathetic innervation is heterogeneous across both the epicardial and endocardial surfaces of the heart.

Original languageEnglish (US)
Pages (from-to)1113-1119
Number of pages7
JournalHeart Rhythm
Volume7
Issue number8
DOIs
StatePublished - Sep 24 2010
Externally publishedYes

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Endocardium
Pericardium
Heart Atria
Swine
Heart Ventricles
Cholinergic Agents
Acetylthiocholine
Parasympathetic Nervous System
Acetylcholinesterase
Nerve Fibers
Coloring Agents
Arteries

Keywords

  • Acetylcholine
  • Nerve density
  • Parasympathetic innervation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Ulphani, J. S., Cain, J. H., Inderyas, F., Gordon, D., Gikas, P. V., Shade, G., ... Goldberger, J. (2010). Quantitative analysis of parasympathetic innervation of the porcine heart. Heart Rhythm, 7(8), 1113-1119. https://doi.org/10.1016/j.hrthm.2010.03.043

Quantitative analysis of parasympathetic innervation of the porcine heart. / Ulphani, Joseph S.; Cain, Jack H.; Inderyas, Firdous; Gordon, David; Gikas, Peter V.; Shade, Gregory; Mayor, David; Arora, Rishi; Kadish, Alan H.; Goldberger, Jeffrey.

In: Heart Rhythm, Vol. 7, No. 8, 24.09.2010, p. 1113-1119.

Research output: Contribution to journalArticle

Ulphani, JS, Cain, JH, Inderyas, F, Gordon, D, Gikas, PV, Shade, G, Mayor, D, Arora, R, Kadish, AH & Goldberger, J 2010, 'Quantitative analysis of parasympathetic innervation of the porcine heart', Heart Rhythm, vol. 7, no. 8, pp. 1113-1119. https://doi.org/10.1016/j.hrthm.2010.03.043
Ulphani JS, Cain JH, Inderyas F, Gordon D, Gikas PV, Shade G et al. Quantitative analysis of parasympathetic innervation of the porcine heart. Heart Rhythm. 2010 Sep 24;7(8):1113-1119. https://doi.org/10.1016/j.hrthm.2010.03.043
Ulphani, Joseph S. ; Cain, Jack H. ; Inderyas, Firdous ; Gordon, David ; Gikas, Peter V. ; Shade, Gregory ; Mayor, David ; Arora, Rishi ; Kadish, Alan H. ; Goldberger, Jeffrey. / Quantitative analysis of parasympathetic innervation of the porcine heart. In: Heart Rhythm. 2010 ; Vol. 7, No. 8. pp. 1113-1119.
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abstract = "Background: Parasympathetic control of the heart is an important component in the regulation of normal cardiac function. However, the anatomic course of parasympathetic innervation of the heart is unclear. Objective: The purpose of this study was to apply a gross parasympathetic nerve stain technique to reveal the details of the morphology of the cardiac parasympathetic nervous system. Methods: Ten whole pig hearts were stained using a histochemical method. Immediately after sacrifice, hearts were placed in a buffered solution containing acetylthiocholine, which precipitates with acetylcholinesterase, allowing identification of cholinergic nerves. The epicardial and endocardial surfaces of the atria and ventricles were examined for nerve thickness and density. Results: In both atria, nerve density was significantly greater on the endocardium, but nerve thickness was significantly greater on the epicardium. The right atrium (RA) was more densely innervated than the left atrium (LA) on the endocardium, whereas the LA was more densely innervated than the RA on the epicardium. In the ventricles, numerous thick cholinergic nerves were clearly identifiable across the epicardium, generally running parallel to the left anterior descending artery. The endocardial surfaces of the ventricles revealed a dense network of fine parasympathetic nerve fibers. As in the atria, nerve density was greater on the ventricular endocardium, but nerve thickness was greater on the epicardium. The right ventricle (RV) was more densely innervated than the left ventricle (LV), whereas the LV endocardium was more densely innervated than the RV endocardium. Conclusion: The epicardial and endocardial surfaces of the atria and ventricles are richly innervated by parasympathetic nerves. The density of parasympathetic innervation is heterogeneous across both the epicardial and endocardial surfaces of the heart.",
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AU - Shade, Gregory

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N2 - Background: Parasympathetic control of the heart is an important component in the regulation of normal cardiac function. However, the anatomic course of parasympathetic innervation of the heart is unclear. Objective: The purpose of this study was to apply a gross parasympathetic nerve stain technique to reveal the details of the morphology of the cardiac parasympathetic nervous system. Methods: Ten whole pig hearts were stained using a histochemical method. Immediately after sacrifice, hearts were placed in a buffered solution containing acetylthiocholine, which precipitates with acetylcholinesterase, allowing identification of cholinergic nerves. The epicardial and endocardial surfaces of the atria and ventricles were examined for nerve thickness and density. Results: In both atria, nerve density was significantly greater on the endocardium, but nerve thickness was significantly greater on the epicardium. The right atrium (RA) was more densely innervated than the left atrium (LA) on the endocardium, whereas the LA was more densely innervated than the RA on the epicardium. In the ventricles, numerous thick cholinergic nerves were clearly identifiable across the epicardium, generally running parallel to the left anterior descending artery. The endocardial surfaces of the ventricles revealed a dense network of fine parasympathetic nerve fibers. As in the atria, nerve density was greater on the ventricular endocardium, but nerve thickness was greater on the epicardium. The right ventricle (RV) was more densely innervated than the left ventricle (LV), whereas the LV endocardium was more densely innervated than the RV endocardium. Conclusion: The epicardial and endocardial surfaces of the atria and ventricles are richly innervated by parasympathetic nerves. The density of parasympathetic innervation is heterogeneous across both the epicardial and endocardial surfaces of the heart.

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