Quantitative analysis of long-term virus-specific CD8+-T-cell memory in mice challenged with unrelated pathogens

Haiyan Liu, Samita Andreansky, Gabriela Diaz, Stephen J. Turner, Dominik Wodarz, Peter C. Doherty

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The consequences for the long-term maintenance of virus-specific CD8+-T-cell memory have been analyzed experimentally for sequential respiratory infections with readily eliminated (influenza virus) and persistent (gammaherpesvirus 68 [γHV681]) pathogens. Sampling a broad range of tissue sites established that the numbers of CD8+ T cells specific for the prominent influenza virus DbNP366 epitope were reduced by about half in mice that had been challenged 100 days previously with γHV68, though the prior presence of a large CD8+ DbNP366+ population caused no selective defect in the γHV68-specific CD8+ Kbp79+ response. Conversely, mice that had been primed and boosted to generate substantial γHV68-specific CD8+ Dbp56+ populations did not show any decrease in prevalence for this set of CD8+ memory cytotoxic T lymphocytes (CTL) at 200 days after respiratory exposure to an influenza A virus. However, in both experiments, the total magnitude of the CD8+-T-cell pool was significantly diminished in those that had been infected with γHV68 and the influenza A virus. The broader implications of these findings, especially under conditions of repeated exposure to unrelated pathogens, are explored with a mathematical model which emphasizes that the immune effector and memory "phenome" is a function of the overall infection experience of the individual.

Original languageEnglish
Pages (from-to)7756-7763
Number of pages8
JournalJournal of Virology
Volume77
Issue number14
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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quantitative analysis
T-lymphocytes
Influenza A virus
Orthomyxoviridae
Viruses
T-Lymphocytes
viruses
pathogens
mice
cytotoxic T-lymphocytes
Cytotoxic T-Lymphocytes
Respiratory Tract Infections
infection
Population
epitopes
Epitopes
Theoretical Models
mathematical models
Infection
sampling

ASJC Scopus subject areas

  • Immunology

Cite this

Quantitative analysis of long-term virus-specific CD8+-T-cell memory in mice challenged with unrelated pathogens. / Liu, Haiyan; Andreansky, Samita; Diaz, Gabriela; Turner, Stephen J.; Wodarz, Dominik; Doherty, Peter C.

In: Journal of Virology, Vol. 77, No. 14, 01.07.2003, p. 7756-7763.

Research output: Contribution to journalArticle

Liu, Haiyan ; Andreansky, Samita ; Diaz, Gabriela ; Turner, Stephen J. ; Wodarz, Dominik ; Doherty, Peter C. / Quantitative analysis of long-term virus-specific CD8+-T-cell memory in mice challenged with unrelated pathogens. In: Journal of Virology. 2003 ; Vol. 77, No. 14. pp. 7756-7763.
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