Quantitative analysis of labelled inner retinal proteins in experimental optic neuritis

John Guy, E. Ann Ellis, Kay Kelley, G. M. Hope, Narsing A. Rao

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


In order to deternine if axonal transport changes in chronic experimental allergic enceph-alomyelitis (EAE) were due to blockade or increased disdwqe of fast transported proteins from the inner retina, we examined the presence of pulse labeled proteins in autoradiograms of the optic nerve head, retinal ganglion cell and nerve fiber layers of juvenile strain-13 guinea pigs with chronic EAE and normal controls. Quantitative analysis of silver grains, performed six and twenty-four hours following the intravitreal injection of tritiated leucine, showed a decrease in inner retinal radioactivity in those with EAE, whereas no difference was detected between the two groups after three days. Grain counts within the optic nerve heads of guinea pigs with EAE were reduced at all time intervals studied. These results are consistent with an increase in discharge of fast transported proteins from retinal ganglion cells into optic nerve axons and support our previous observations of increased radioactivity at the foci of optic nerve demyelination.

Original languageEnglish (US)
Pages (from-to)253-260
Number of pages8
JournalCurrent Eye Research
Issue number3
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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