Quantitation of malaria sporozoites transmitted in vitro during salivation by wild Afrotropical Anopheles

JOHN C. BEIER, FRED K. ONYANGO, JOSEPH K. KOROS, MUTALIB RAMADHAN, ROSE OGWANG, ROBERT A. WIRTZ, DAVY K. KOECH, CLIFFORD R. ROBERTS

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

The malaria transmission potential of wild, infective Anopheles from western Kenya was evaluated by determining the number of sporozoites transmitted in vitro by salivation when their mouthparts were inserted into capillary tubes containing either sucrose or blood. With sucrose, 86.6% of 102 infective Anopheles transmitted a geometric mean (GM) of 3.84 sporozoites (range 1-34). With blood, 23.1% of 104 infective Anopheles, tested on the day of collection, transmitted a GM of 2.30 sporozoites (range 1-117). For Anopheles held 5 days postcapture before testing with blood, 53.6% of 56 transmitted a GM of 6.04 sporozoites (range 1-420). Transmitting Anopheles contained significantly more salivary gland sporozoites than non-transmitters. No significant differences were detected between Anopheles gambiae Giles sensu lato and Anopheles funestus Giles in sporozoite transmission by individuals with sporozoites in their salivary glands. Sporozoites were detected microscopically in the salivary duct from heads in 80.3% of 117 infective Anopheles (GM = 11.2, range 1-71). Sporozoite detection in mosquito heads by ELISA was 25% less efficient than microscopic detection. Over 98% of the infective Anopheles transmitted less than twenty-five Over 98% of the infective Anopheles transmitted less than twenty-five sporozoites. Transmitted sporozoites represented only about 3% of the total sporozoites in the salivary glands suggesting that sporozoite transmission may be restricted to sporozoites in the salivary duct at the time of feeding. Results are discussed in relation to anti-sporozoite vaccine development.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalMedical and Veterinary Entomology
Volume5
Issue number1
DOIs
StatePublished - Jan 1991
Externally publishedYes

Keywords

  • Anopheles funestus
  • Anopheles gambiae
  • Kenya
  • Malaria
  • sporozoites
  • transmission

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

Fingerprint Dive into the research topics of 'Quantitation of malaria sporozoites transmitted in vitro during salivation by wild Afrotropical Anopheles'. Together they form a unique fingerprint.

  • Cite this

    BEIER, JOHN. C., ONYANGO, FRED. K., KOROS, JOSEPH. K., RAMADHAN, MUTALIB., OGWANG, ROSE., WIRTZ, ROBERT. A., KOECH, DAVY. K., & ROBERTS, CLIFFORD. R. (1991). Quantitation of malaria sporozoites transmitted in vitro during salivation by wild Afrotropical Anopheles. Medical and Veterinary Entomology, 5(1), 71-79. https://doi.org/10.1111/j.1365-2915.1991.tb00523.x