Abstract
The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).
| Original language | English |
|---|---|
| Pages (from-to) | 63-70 |
| Number of pages | 8 |
| Journal | Medical and Veterinary Entomology |
| Volume | 5 |
| Issue number | 1 |
| State | Published - Jan 1 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Insect Science
- veterinary(all)
Cite this
Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles. / Beier, John C; Onyango, F. K.; Ramadhan, M.; Koros, J. K.; Asiago, C. M.; Wirtz, R. A.; Koech, D. K.; Roberts, C. R.
In: Medical and Veterinary Entomology, Vol. 5, No. 1, 01.01.1991, p. 63-70.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles.
AU - Beier, John C
AU - Onyango, F. K.
AU - Ramadhan, M.
AU - Koros, J. K.
AU - Asiago, C. M.
AU - Wirtz, R. A.
AU - Koech, D. K.
AU - Roberts, C. R.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).
AB - The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).
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UR - http://www.scopus.com/inward/citedby.url?scp=0025718123&partnerID=8YFLogxK
M3 - Article
C2 - 1768902
AN - SCOPUS:0025718123
VL - 5
SP - 63
EP - 70
JO - Medical and Veterinary Entomology
JF - Medical and Veterinary Entomology
SN - 0269-283X
IS - 1
ER -