Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles

JOHN C. BEIER, FRED K. ONYANGO, MUTALIB RAMADHAN, JOSEPH K. KOROS, CHARLES M. ASIAGO, ROBERT A. WIRTZ, DAVY K. KOECH, CLIFFORD R. ROBERTS

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalMedical and Veterinary Entomology
Volume5
Issue number1
DOIs
StatePublished - Jan 1991
Externally publishedYes

Keywords

  • Anopheles funestus
  • Anopheles gambiae
  • ELISA
  • Kenya
  • Malaria
  • quantitation
  • sporozoites

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

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  • Cite this

    BEIER, JOHN. C., ONYANGO, FRED. K., RAMADHAN, MUTALIB., KOROS, JOSEPH. K., ASIAGO, CHARLES. M., WIRTZ, ROBERT. A., KOECH, DAVY. K., & ROBERTS, CLIFFORD. R. (1991). Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles. Medical and Veterinary Entomology, 5(1), 63-70. https://doi.org/10.1111/j.1365-2915.1991.tb00522.x