Abstract
The number of malaria sporozoites in the salivary glands was determined microscopically for 1137 wild, naturally infected Anopheles from western Kenya. Infective Anopheles gambiae Giles sensu lato (n = 874) contained a geometric mean (GM) of 962 sporozoites and An.funestus Giles (n = 263) contained 812. No significant differences were detected in geometric mean numbers of sporozoites between species, collection techniques or sites. Of the infective An.gambiae, 1.7% (15/874) contained more than 41,830 sporozoites, the maximum observed for An.funestus. Microscopic techniques were found to be more sensitive than enzyme-linked immunosorbent assays (ELISA) for detecting low-grade sporozoite infections in salivary glands. Salivary gland sporozoites from 83.6% of the 1137 gland infections were identified by ELISA as either Plasmodium falciparum Welch (n = 910), P.ovale Stephens (n = 7), P.malariae Grassi & Feletti (n = 3) or mixed (n = 30). The 187 gland infections which could not be identified by ELISA contained significantly fewer sporozoites (GM = 242) than those which could be identified (GM = 1200).
Original language | English (US) |
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Pages (from-to) | 63-70 |
Number of pages | 8 |
Journal | Medical and Veterinary Entomology |
Volume | 5 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1991 |
Externally published | Yes |
Keywords
- Anopheles funestus
- Anopheles gambiae
- ELISA
- Kenya
- Malaria
- quantitation
- sporozoites
ASJC Scopus subject areas
- Parasitology
- Ecology, Evolution, Behavior and Systematics
- veterinary(all)
- Insect Science