Quantitation of HIV-1 RNA levels in plasma and CSF of asymptomatic HIV-1 infected patients from South India using a TaqMan real time PCR assay

Anupa Kamat, V. Ravi, Anita Desai, P. Satishchandra, K. S. Satish, I. Borodowsky, D. K. Subbakrishna, Mahendra Kumar

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background: Most of the quantitation assays for HIV-1 RNA used currently are designed and optimized for HIV-1 subtype B viruses and hence may not be suitable for India, where the predominant subtype is HIV-1 subtype C. Objectives: Development and standardization of HIV-1 TaqMan real time PCR assay suitable for measuring plasma and CSF viral RNA levels in HIV subtype C infected individuals. Study design: A TaqMan real time PCR was developed using primers and probes selected in the gag region for detection of Indian HIV-1 subtype C strain. Plasma (n = 120) and CSF samples (n = 46) obtained from HIV infected subjects were used to evaluate the sensitivity and specificity of the assay. A comparative evaluation was carried out with a commercially available quantitative HIV viral load assay (Roche Amplicor Version 1.5). Results: The TaqMan assay was able to amplify all HIV-1 group M subtypes except subtype E. Viral loads could be estimated in all the plasma (n = 120) and 40/46 CSF samples obtained from HIV positive subjects. Sensitivity of this assay was found to be 180 copies/ml. Correlation with the commercially available viral load assay was very good (r = 0.885). Conclusions: A TaqMan real time PCR was standardized for HIV-1 subtype C and it was more sensitive (180 copies/ml) than standard Amplicor monitor assay, Version 1.5 (400 copies/ml).

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalJournal of Clinical Virology
Volume39
Issue number1
DOIs
StatePublished - May 1 2007

Keywords

  • HIV infection in South India
  • HIV-1 TaqMan viral load assay
  • Plasma and CSF viral loads

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

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