Abstract
The enzyme-linked immunosorbent assay (ELISA) for candida polysaccharide antigen in serum was developed by using double antibody sandwich technique. It was found that serum constituents substantially interfered with the assay. The interference was successfully decreased by heating serum in the presence of EDTA which precipitated interfering serum protein. In addition, candida antigen may be liberated from antibody complexes. The candida polysaccharide antigen in the supernatant was precipitated with ethanol and resuspended in the buffer containing gelatin. A maximum sensitivity of 10 ng/ml was found in the assay using this procedure. This procedure of serum treatment may have potential applications for the detection of other polysaccharide antigens in serum.
| Original language | English |
|---|---|
| Pages (from-to) | 155-163 |
| Number of pages | 9 |
| Journal | Research Communications in Chemical Pathology and Pharmacology |
| Volume | 35 |
| Issue number | 1 |
| State | Published - Jan 1 1982 |
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ASJC Scopus subject areas
- Pharmacology
- Toxicology
Cite this
Quantitation of Candida albicans polysaccharide using an enzyme-linked immunosorbent assay. / Cleary, Timothy; Monji, T.; Parker, J.; Castro, A.
In: Research Communications in Chemical Pathology and Pharmacology, Vol. 35, No. 1, 01.01.1982, p. 155-163.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitation of Candida albicans polysaccharide using an enzyme-linked immunosorbent assay
AU - Cleary, Timothy
AU - Monji, T.
AU - Parker, J.
AU - Castro, A.
PY - 1982/1/1
Y1 - 1982/1/1
N2 - The enzyme-linked immunosorbent assay (ELISA) for candida polysaccharide antigen in serum was developed by using double antibody sandwich technique. It was found that serum constituents substantially interfered with the assay. The interference was successfully decreased by heating serum in the presence of EDTA which precipitated interfering serum protein. In addition, candida antigen may be liberated from antibody complexes. The candida polysaccharide antigen in the supernatant was precipitated with ethanol and resuspended in the buffer containing gelatin. A maximum sensitivity of 10 ng/ml was found in the assay using this procedure. This procedure of serum treatment may have potential applications for the detection of other polysaccharide antigens in serum.
AB - The enzyme-linked immunosorbent assay (ELISA) for candida polysaccharide antigen in serum was developed by using double antibody sandwich technique. It was found that serum constituents substantially interfered with the assay. The interference was successfully decreased by heating serum in the presence of EDTA which precipitated interfering serum protein. In addition, candida antigen may be liberated from antibody complexes. The candida polysaccharide antigen in the supernatant was precipitated with ethanol and resuspended in the buffer containing gelatin. A maximum sensitivity of 10 ng/ml was found in the assay using this procedure. This procedure of serum treatment may have potential applications for the detection of other polysaccharide antigens in serum.
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M3 - Article
VL - 35
SP - 155
EP - 163
JO - Research Communications in Molecular Pathology and Pharmacology
JF - Research Communications in Molecular Pathology and Pharmacology
SN - 0034-5164
IS - 1
ER -