Quantifying the performance of protein-resisting surfaces at ultra-low protein coverages using kinesin motor proteins as probes

Parag Katira, Ashutosh Agarwal, Thorsten Fischer, Hsien Yeh Chen, Xuwei Jiang, Joerg Lahann, Henry Hess

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The quantification of ultra-low kinesin converges by landing rate measurements was demonstrated for the determination of coatings performance and their resistance to protein absorption. The surface concentration of adsorbed kinesin motors after a defined period was quantified by measuring the initial landing rate R of microtubules on the coated surface and the diffusion-limited landing rate Z on a control surface densely coated with motor proteins. The results show that the direct adsorption of kinesin to fouling and moderately non-fouling surface leads to denaturation and loss of its microtubule-binding ability. Direct adsorption of kinesin to highly non-fouling surface leads to low landing rates that do not increase with increasing kinesin concentration in solution. The results also show that the highly no-fouling surface conserves kinesin motor function after adsorption and that casein acts as a competitor fro a very limited number of adsorption sites.

Original languageEnglish (US)
Pages (from-to)3171-3176
Number of pages6
JournalAdvanced Materials
Volume19
Issue number20
DOIs
StatePublished - Oct 19 2007
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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