Quantifying and understanding protein adsorption to non-fouling surfaces

Ashutosh Agarwal; Parag Katira; Henry Hess

doi:10.1109/NEBC.2010.5458217

Quantifying and understanding protein adsorption to non-fouling surfaces

Ashutosh Agarwal, Parag Katira, Henry Hess

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Surfaces grafted with poly(ethylene oxide) (PEO) are known to resist protein adsorption. Research efforts in this field have focused on both developing surfaces with better resistance to protein adsorption and understanding the origin of resistance of PEO grafted surfaces to protein adsorption. In the first part of this contribution, we describe a novel quantification technique for extremely low protein coverage on surfaces. This technique utilizes measurement of the landing rate of microtubule filaments on kinesin proteins adsorbed on a surface to determine the kinesin density. The detection limit of our technique is 100 times lower than that of standard characterization methods and is employed to test the performance of novel and established coatings with outstanding resistance to protein adsorption. In the second part, a random sequential adsorption (RSA) model is presented for protein adsorption to PEO coated surfaces. The model suggests that PEO chains act as almost perfect steric barriers to protein adsorption. Furthermore, it can be used to predict the performance of a variety of systems towards resisting protein adsorption and can help in the design of better nonfouling surface coatings.

Original language	English (US)
Title of host publication	Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010
DOIs	https://doi.org/10.1109/NEBC.2010.5458217
State	Published - Jun 8 2010
Externally published	Yes
Event	36th Annual Northeast Bioengineering Conference, NEBEC 2010 - New York, NY, United States Duration: Mar 26 2010 → Mar 28 2010

Publication series

Name	Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010

Other

Other	36th Annual Northeast Bioengineering Conference, NEBEC 2010
Country	United States
City	New York, NY
Period	3/26/10 → 3/28/10

ASJC Scopus subject areas

Bioengineering

Access to Document

10.1109/NEBC.2010.5458217

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Agarwal, A., Katira, P., & Hess, H. (2010). Quantifying and understanding protein adsorption to non-fouling surfaces. In Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010 [5458217] (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010). https://doi.org/10.1109/NEBC.2010.5458217

Quantifying and understanding protein adsorption to non-fouling surfaces. / Agarwal, Ashutosh; Katira, Parag; Hess, Henry.

Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010. 2010. 5458217 (Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Agarwal, A, Katira, P & Hess, H 2010, Quantifying and understanding protein adsorption to non-fouling surfaces. in Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010., 5458217, Proceedings of the 2010 IEEE 36th Annual Northeast Bioengineering Conference, NEBEC 2010, 36th Annual Northeast Bioengineering Conference, NEBEC 2010, New York, NY, United States, 3/26/10. https://doi.org/10.1109/NEBC.2010.5458217

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