Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho- ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellularlevel responses to targeted anti-tumor therapeutics.
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