Quantified immunohistochemistry demonstrates cellular level targets of an oral multikinase inhibitor in metastatic thyroid cancer

Evan R. Ransom, Jason Leibowitz, Cathy Ma, Kanchan Puttaswamy, Andrea Troxel, William Lee, Michael Feldman, Marcia S. Brose

Research output: Contribution to journalArticle

Abstract

Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho- ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellularlevel responses to targeted anti-tumor therapeutics.

Original languageEnglish
JournalLaryngoscope
Volume120
Issue numberSUPPL. 3
DOIs
StatePublished - Oct 1 2010
Externally publishedYes

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Thyroid Neoplasms
Immunohistochemistry
Staining and Labeling
Phase II Clinical Trials
Therapeutic Uses
Therapeutics
Tumor Biomarkers
Neoplasms

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Quantified immunohistochemistry demonstrates cellular level targets of an oral multikinase inhibitor in metastatic thyroid cancer. / Ransom, Evan R.; Leibowitz, Jason; Ma, Cathy; Puttaswamy, Kanchan; Troxel, Andrea; Lee, William; Feldman, Michael; Brose, Marcia S.

In: Laryngoscope, Vol. 120, No. SUPPL. 3, 01.10.2010.

Research output: Contribution to journalArticle

Ransom, Evan R. ; Leibowitz, Jason ; Ma, Cathy ; Puttaswamy, Kanchan ; Troxel, Andrea ; Lee, William ; Feldman, Michael ; Brose, Marcia S. / Quantified immunohistochemistry demonstrates cellular level targets of an oral multikinase inhibitor in metastatic thyroid cancer. In: Laryngoscope. 2010 ; Vol. 120, No. SUPPL. 3.
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AU - Ma, Cathy

AU - Puttaswamy, Kanchan

AU - Troxel, Andrea

AU - Lee, William

AU - Feldman, Michael

AU - Brose, Marcia S.

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