Abstract
Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho- ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellularlevel responses to targeted anti-tumor therapeutics.
| Original language | English |
|---|---|
| Journal | Laryngoscope |
| Volume | 120 |
| Issue number | SUPPL. 3 |
| DOIs | |
| State | Published - Oct 1 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Quantified immunohistochemistry demonstrates cellular level targets of an oral multikinase inhibitor in metastatic thyroid cancer. / Ransom, Evan R.; Leibowitz, Jason; Ma, Cathy; Puttaswamy, Kanchan; Troxel, Andrea; Lee, William; Feldman, Michael; Brose, Marcia S.
In: Laryngoscope, Vol. 120, No. SUPPL. 3, 01.10.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantified immunohistochemistry demonstrates cellular level targets of an oral multikinase inhibitor in metastatic thyroid cancer
AU - Ransom, Evan R.
AU - Leibowitz, Jason
AU - Ma, Cathy
AU - Puttaswamy, Kanchan
AU - Troxel, Andrea
AU - Lee, William
AU - Feldman, Michael
AU - Brose, Marcia S.
PY - 2010/10/1
Y1 - 2010/10/1
N2 - Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho- ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellularlevel responses to targeted anti-tumor therapeutics.
AB - Objectives: To describe a new technique for assaying effects of antitumor therapeutics over time. Study Design: Tumor tissue samples were taken from a Phase II clinical trial. A method for quantification of immuno-histochemistry (IHC) was developed using a computer algorithm designed to measure staining levels spectrographically. Phospho- ERK was selected as the tumor marker for this demonstration. Results: Average nuclear DAB intensities were 846.972 (pre-treatment) and 229.249 (on-treatment). Staining intensity frequency distribution curves generated showed statistically distinct distributions, both confirming and quantifying the decreased presence of phospho-ERK in the on-treatment sample. Conclusions: A novel technique for quantification of IHC is presented. This process can be used to delineate specific cellularlevel responses to targeted anti-tumor therapeutics.
UR - http://www.scopus.com/inward/record.url?scp=78651434009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651434009&partnerID=8YFLogxK
U2 - 10.1002/lary.21231
DO - 10.1002/lary.21231
M3 - Article
VL - 120
JO - Laryngoscope
JF - Laryngoscope
SN - 0023-852X
IS - SUPPL. 3
ER -