Quantification of uptake of liposomal carboxyfluorescein by professional phagocytes in-vitro. A flow microfluorimetric study on the J774 murine macrophage cell line

Mario Stevenson, A. J. Baillie, R. M E Richards

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Unilamellar egg phosphatidylcholine/cholesterol liposomes containing carboxyfluorescein were prepared by an ether injection method. The ability of cells of the J774.2 murine macrophage cell line to incorporate the liposomal fluorophore during incubation at 37° C was measured by flow microfluorimetry. Liposomes incorporating additional phosphatidylserine or phosphatidic acid were taken up much more avidly than those lacking these phospholipids and the greatest uptake of carboxyfluorescein was observed with the phosphatidylserine species. Calculation of the number of liposomes taken up, ≱0.2% of the number given, showed that this was an inefficient process. However these uptake data support previous findings based on the intracellular bactericidal activity of liposomal antibiotics determined in an identical in-vitro system.

Original languageEnglish
Pages (from-to)824-830
Number of pages7
JournalJournal of Pharmacy and Pharmacology
Volume36
Issue number12
StatePublished - Dec 1 1984
Externally publishedYes

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Phagocytes
Liposomes
Phosphatidylserines
Macrophages
Cell Line
Phosphatidic Acids
Phosphatidylcholines
Ether
Ovum
Phospholipids
Flow Cytometry
Cholesterol
Anti-Bacterial Agents
Injections
6-carboxyfluorescein
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

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abstract = "Unilamellar egg phosphatidylcholine/cholesterol liposomes containing carboxyfluorescein were prepared by an ether injection method. The ability of cells of the J774.2 murine macrophage cell line to incorporate the liposomal fluorophore during incubation at 37° C was measured by flow microfluorimetry. Liposomes incorporating additional phosphatidylserine or phosphatidic acid were taken up much more avidly than those lacking these phospholipids and the greatest uptake of carboxyfluorescein was observed with the phosphatidylserine species. Calculation of the number of liposomes taken up, ≱0.2{\%} of the number given, showed that this was an inefficient process. However these uptake data support previous findings based on the intracellular bactericidal activity of liposomal antibiotics determined in an identical in-vitro system.",
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T1 - Quantification of uptake of liposomal carboxyfluorescein by professional phagocytes in-vitro. A flow microfluorimetric study on the J774 murine macrophage cell line

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AU - Baillie, A. J.

AU - Richards, R. M E

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N2 - Unilamellar egg phosphatidylcholine/cholesterol liposomes containing carboxyfluorescein were prepared by an ether injection method. The ability of cells of the J774.2 murine macrophage cell line to incorporate the liposomal fluorophore during incubation at 37° C was measured by flow microfluorimetry. Liposomes incorporating additional phosphatidylserine or phosphatidic acid were taken up much more avidly than those lacking these phospholipids and the greatest uptake of carboxyfluorescein was observed with the phosphatidylserine species. Calculation of the number of liposomes taken up, ≱0.2% of the number given, showed that this was an inefficient process. However these uptake data support previous findings based on the intracellular bactericidal activity of liposomal antibiotics determined in an identical in-vitro system.

AB - Unilamellar egg phosphatidylcholine/cholesterol liposomes containing carboxyfluorescein were prepared by an ether injection method. The ability of cells of the J774.2 murine macrophage cell line to incorporate the liposomal fluorophore during incubation at 37° C was measured by flow microfluorimetry. Liposomes incorporating additional phosphatidylserine or phosphatidic acid were taken up much more avidly than those lacking these phospholipids and the greatest uptake of carboxyfluorescein was observed with the phosphatidylserine species. Calculation of the number of liposomes taken up, ≱0.2% of the number given, showed that this was an inefficient process. However these uptake data support previous findings based on the intracellular bactericidal activity of liposomal antibiotics determined in an identical in-vitro system.

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