TY - JOUR
T1 - Quantification of Nucleic Acid Concentration in the Nanoparticle or Polymer Conjugates Using Circular Dichroism Spectroscopy
AU - Peng, Zhili
AU - Li, Jiaojiao
AU - Li, Shanghao
AU - Pardo, Joel
AU - Zhou, Yiqun
AU - Al-Youbi, Abdulrahman O.
AU - Bashammakh, Abdulaziz S.
AU - El-Shahawi, Mohammad S.
AU - Leblanc, Roger M.
N1 - Funding Information:
R.M.L. gratefully acknowledges the support from King Abdulaziz University, Saudi Arabia, the University of Miami, USA, and the National Institute of Health grant R21 AR072226.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/2/6
Y1 - 2018/2/6
N2 - The interface of nucleic acids and nanomaterials is among the most promising fields in recent years. Considerable efforts have been devoted to the development of novel systems based on the two components for various promising applications such as sensing, bioimaging, drug delivery, and theranostics. However, the determination of nucleic acid concentration in these systems remains as a challenge due to the interference of nanoparticles. To this end, we developed a simple, yet reliable, method to quantify the nucleic acid concentration in their nanoparticle or polymer conjugates based on circular dichroism (CD) spectroscopy. In this paper, three nucleic acids, namely, DNA sodium salt from calf thymus (NaDNA), DNA from herring sperm (hsDNA), and ribonucleic acid from torula yeast (tyRNA), were noncovalently conjugated to three nanoparticles. The concentrations of the three nucleic acids in their nanoparticle conjugates were successfully determined on the basis of CD spectra calibration curves.
AB - The interface of nucleic acids and nanomaterials is among the most promising fields in recent years. Considerable efforts have been devoted to the development of novel systems based on the two components for various promising applications such as sensing, bioimaging, drug delivery, and theranostics. However, the determination of nucleic acid concentration in these systems remains as a challenge due to the interference of nanoparticles. To this end, we developed a simple, yet reliable, method to quantify the nucleic acid concentration in their nanoparticle or polymer conjugates based on circular dichroism (CD) spectroscopy. In this paper, three nucleic acids, namely, DNA sodium salt from calf thymus (NaDNA), DNA from herring sperm (hsDNA), and ribonucleic acid from torula yeast (tyRNA), were noncovalently conjugated to three nanoparticles. The concentrations of the three nucleic acids in their nanoparticle conjugates were successfully determined on the basis of CD spectra calibration curves.
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U2 - 10.1021/acs.analchem.7b04621
DO - 10.1021/acs.analchem.7b04621
M3 - Article
C2 - 29281251
AN - SCOPUS:85041423301
VL - 90
SP - 2255
EP - 2262
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 3
ER -