Quantification of Nucleic Acid Concentration in the Nanoparticle or Polymer Conjugates Using Circular Dichroism Spectroscopy

Zhili Peng, Jiaojiao Li, Shanghao Li, Joel Pardo, Yiqun Zhou, Abdulrahman O. Al-Youbi, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Roger Leblanc

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The interface of nucleic acids and nanomaterials is among the most promising fields in recent years. Considerable efforts have been devoted to the development of novel systems based on the two components for various promising applications such as sensing, bioimaging, drug delivery, and theranostics. However, the determination of nucleic acid concentration in these systems remains as a challenge due to the interference of nanoparticles. To this end, we developed a simple, yet reliable, method to quantify the nucleic acid concentration in their nanoparticle or polymer conjugates based on circular dichroism (CD) spectroscopy. In this paper, three nucleic acids, namely, DNA sodium salt from calf thymus (NaDNA), DNA from herring sperm (hsDNA), and ribonucleic acid from torula yeast (tyRNA), were noncovalently conjugated to three nanoparticles. The concentrations of the three nucleic acids in their nanoparticle conjugates were successfully determined on the basis of CD spectra calibration curves.

Original languageEnglish (US)
Pages (from-to)2255-2262
Number of pages8
JournalAnalytical Chemistry
Volume90
Issue number3
DOIs
StatePublished - Feb 6 2018

ASJC Scopus subject areas

  • Analytical Chemistry

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