TY - JOUR
T1 - Quantification of Choriocapillaris with Optical Coherence Tomography Angiography
T2 - A Comparison Study
AU - Chu, Zhongdi
AU - Gregori, Giovanni
AU - Rosenfeld, Philip J.
AU - Wang, Ruikang K.
N1 - Funding Information:
All authors have completed and submitted the ICMJE form for Disclosure of Potential Conflicts of Interest and none were reported. Financial Disclosures: Drs. Gregori, Wang, and Rosenfeld have received research support from Carl Zeiss Meditec . Dr. Gregori and University of Miami co-own a patent licensed to Carl Zeiss Meditec, Inc. Dr. Rosenfeld is a consultant for Apellis, Boehringer Ingelheim, Carl Zeiss Meditec, Chengdu Kanghong Biotech, Ocunexus Therapeutics, Genentech, Healios KK, Hemera Biosciences, F. Hoffmann-La Roche Ltd., Isarna Pharmaceuticals, Ocunexus, Ocudyne, and Unity Biotechnology; and holds equity in Apellis, Verana Health, and Ocudyne. Dr. Wang holds intellectual property owned by the Oregon Health and Science University and the University of Washington; and has received research support from Tasso Inc. , Moptim Inc. , Colgate Palmolive Co. , and Facebook LLC ; and is a consultant for Insight Photonic Solutions, Kowa, and Carl Zeiss Meditec. All other authors indicate no financial support or financial conflict of interest. Funding/Support: Research has been supported by US National Eye Institute grants R01EY024158 and R01EY028753 , National Eye Institute Center core grant P30EY014801 , the Salah Foundation , Carl Zeiss Meditec , an unrestricted grant from the Research to Prevent Blindness , Inc., and the Department of Ophthalmology, University of Miami Miller School of Medicine . The funding organization had no role in the design or conduct of this research.
Funding Information:
All authors have completed and submitted the ICMJE form for Disclosure of Potential Conflicts of Interest and none were reported. Financial Disclosures: Drs. Gregori, Wang, and Rosenfeld have received research support from Carl Zeiss Meditec. Dr. Gregori and University of Miami co-own a patent licensed to Carl Zeiss Meditec, Inc. Dr. Rosenfeld is a consultant for Apellis, Boehringer Ingelheim, Carl Zeiss Meditec, Chengdu Kanghong Biotech, Ocunexus Therapeutics, Genentech, Healios KK, Hemera Biosciences, F. Hoffmann-La Roche Ltd. Isarna Pharmaceuticals, Ocunexus, Ocudyne, and Unity Biotechnology; and holds equity in Apellis, Verana Health, and Ocudyne. Dr. Wang holds intellectual property owned by the Oregon Health and Science University and the University of Washington; and has received research support from Tasso Inc., Moptim Inc., Colgate Palmolive Co., and Facebook LLC; and is a consultant for Insight Photonic Solutions, Kowa, and Carl Zeiss Meditec. All other authors indicate no financial support or financial conflict of interest. Funding/Support: Research has been supported by US National Eye Institute grants R01EY024158 and R01EY028753, National Eye Institute Center core grant P30EY014801, the Salah Foundation, Carl Zeiss Meditec, an unrestricted grant from the Research to Prevent Blindness, Inc. and the Department of Ophthalmology, University of Miami Miller School of Medicine. The funding organization had no role in the design or conduct of this research.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/12
Y1 - 2019/12
N2 - Purpose: To demonstrate the variation in quantitative choriocapillaris (CC) metrics with various binarization approaches using optical coherence tomography angiography (OCTA). Design: Retrospective, observational, cross-sectional case series. Methods: Macular OCTA scans, 3- × 3-mm and 6- × 6-mm, were obtained from normal eyes and from eyes with drusen secondary to age-related macular degeneration (AMD). The CC slab was extracted, and the CC flow deficits (FDs) were segmented with 2 previously published algorithms: the fuzzy C-means approach (FCM method) and Phansalkar's local thresholding (Phansalkar method). Four different values for the radius were used in order to investigate the effect on the FD segmentation when using the Phansalkar method. FD density (FDD), mean FD size (MFDS), FD number (FDN), FD area (FDA) and intercapillary distance (ICD) were calculated for comparison. Repeatability was assessed as coefficient of variation (CV), and Pearson's correlation analysis was conducted. Results: Six eyes from 6 subjects with normal eyes and 6 eyes from 6 subjects with drusen secondary to AMD were scanned. The 3- × 3-mm scans resulted in higher repeatability than the 6- × 6-mm scans. For the Phansalkar method, larger values of the radius resulted in higher repeatability. ANOVA tests resulted in significant differences (P < 0.001) among the FCM method and the Phansalkar method with different radius options for all CC metrics and scan sizes investigated. In 3- × 3-mm scans, significant correlation was found between the FCM method and the Phansalkar method for all quantitative CC metrics other than FDN (all P < 0.001; 0.90 < r <0.99). Conclusions: Quantitative CC analysis with commercially available OCTA is complicated and researchers need to pay close attention to how they conduct such analyses.
AB - Purpose: To demonstrate the variation in quantitative choriocapillaris (CC) metrics with various binarization approaches using optical coherence tomography angiography (OCTA). Design: Retrospective, observational, cross-sectional case series. Methods: Macular OCTA scans, 3- × 3-mm and 6- × 6-mm, were obtained from normal eyes and from eyes with drusen secondary to age-related macular degeneration (AMD). The CC slab was extracted, and the CC flow deficits (FDs) were segmented with 2 previously published algorithms: the fuzzy C-means approach (FCM method) and Phansalkar's local thresholding (Phansalkar method). Four different values for the radius were used in order to investigate the effect on the FD segmentation when using the Phansalkar method. FD density (FDD), mean FD size (MFDS), FD number (FDN), FD area (FDA) and intercapillary distance (ICD) were calculated for comparison. Repeatability was assessed as coefficient of variation (CV), and Pearson's correlation analysis was conducted. Results: Six eyes from 6 subjects with normal eyes and 6 eyes from 6 subjects with drusen secondary to AMD were scanned. The 3- × 3-mm scans resulted in higher repeatability than the 6- × 6-mm scans. For the Phansalkar method, larger values of the radius resulted in higher repeatability. ANOVA tests resulted in significant differences (P < 0.001) among the FCM method and the Phansalkar method with different radius options for all CC metrics and scan sizes investigated. In 3- × 3-mm scans, significant correlation was found between the FCM method and the Phansalkar method for all quantitative CC metrics other than FDN (all P < 0.001; 0.90 < r <0.99). Conclusions: Quantitative CC analysis with commercially available OCTA is complicated and researchers need to pay close attention to how they conduct such analyses.
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U2 - 10.1016/j.ajo.2019.07.003
DO - 10.1016/j.ajo.2019.07.003
M3 - Article
C2 - 31323202
AN - SCOPUS:85071402603
VL - 208
SP - 111
EP - 123
JO - American Journal of Ophthalmology
JF - American Journal of Ophthalmology
SN - 0002-9394
ER -