Quality control of ribosomal RNA mediated by polynucleotide phosphorylase and RNase R

Zhuan Fen Cheng, Murray P Deutscher

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Despite their overall accuracy, errors in macromolecular processes, such as rRNA synthesis and ribosome assembly, inevitably occur. However, whether these errors are remediated and how this might be accomplished is not known. In previous work, we showed that a double mutant strain lacking both polynucleotide phosphorylase (PNPase) and RNase R activities is inviable. In the course of examining the molecular basis for this phenotype, we found that shifting a temperature-sensitive mutant strain to 42°C led to cessation of growth and loss of cell viability. Northern analysis of RNA isolated from such cells after the temperature shift revealed that fragments of 16S and 23S rRNA accumulated to a high level, and that the amount of ribosomes and ribosomal subunits decreased due to defects in ribosome assembly. rRNA fragments were not detected at 31°C or when single mutant strains were grown at 42°C. Pulse - chase analysis showed that the rRNA fragments appeared within 5 min at 42°C, and that they accumulated before the loss of cell viability. The data are consistent with a model in which PNPase and RNase R mediate a previously unknown quality control process that normally removes defective rRNAs as soon as they are generated. In the absence of these RNases, rRNA fragments accumulate, leading to interference with ribosome maturation and ultimately to cell death.

Original languageEnglish
Pages (from-to)6388-6393
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
StatePublished - May 27 2003

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Polyribonucleotide Nucleotidyltransferase
Ribosomal RNA
Ribosomes
Quality Control
Ribosome Subunits
Cell Survival
Temperature
Ribonucleases
Cell Death
RNA
Phenotype
Growth
ribonuclease R

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Quality control of ribosomal RNA mediated by polynucleotide phosphorylase and RNase R. / Cheng, Zhuan Fen; Deutscher, Murray P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 11, 27.05.2003, p. 6388-6393.

Research output: Contribution to journalArticle

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