Pyroptotic neuronal cell death mediated by the AIM2 inflammasome

Stephanie E. Adamczak, Juan Pablo P de Rivero Vaccari, Gordon Dale, Frank J. Brand, Doris Nonner, Ross Bullock, Gerhard Dahl, W. Dalton Dietrich, Robert Keane

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The central nervous system (CNS) is an active participant in the innate immune response to infection and injury. In these studies, we show embryonic cortical neurons express a functional, deoxyribonucleic acid (DNA)-responsive, absent in melanoma 2 (AIM2) inflammasome that activates caspase-1. Neurons undergo pyroptosis, a proinflammatory cell death mechanism characterized by the following: (a) oligomerization of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC); (b) caspase-1 dependency; (c) formation of discrete pores in the plasma membrane; and (d) release of the inflammatory cytokine interleukin-1β (IL-1β). Probenecid and Brilliant Blue FCF, inhibitors of the pannexin1 channel, prevent AIM2 inflammasome-mediated cell death, identifying pannexin1 as a cell death effector during pyroptosis and probenecid as a novel pyroptosis inhibitor. Furthermore, we show activation of the AIM2 inflammasome in neurons by cerebrospinal fluid (CSF) from traumatic brain injury (TBI) patients and oligomerization of ASC. These findings suggest neuronal pyroptosis is an important cell death mechanism during CNS infection and injury that may be attenuated by probenecid.

Original languageEnglish
Pages (from-to)621-629
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume34
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Inflammasomes
Probenecid
Melanoma
Cell Death
Caspase 1
Neurons
Nervous System Trauma
Central Nervous System Infections
Interleukin-1
Innate Immunity
Cerebrospinal Fluid
Central Nervous System
Cell Membrane
Apoptosis
Cytokines
Pyroptosis
DNA
Wounds and Injuries
Infection
Proteins

Keywords

  • Brain trauma
  • Cell death
  • Cerebrospinal fluid
  • Inflammasomes
  • Innate immunity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Pyroptotic neuronal cell death mediated by the AIM2 inflammasome. / Adamczak, Stephanie E.; de Rivero Vaccari, Juan Pablo P; Dale, Gordon; Brand, Frank J.; Nonner, Doris; Bullock, Ross; Dahl, Gerhard; Dalton Dietrich, W.; Keane, Robert.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 34, No. 4, 01.01.2014, p. 621-629.

Research output: Contribution to journalArticle

Adamczak, Stephanie E. ; de Rivero Vaccari, Juan Pablo P ; Dale, Gordon ; Brand, Frank J. ; Nonner, Doris ; Bullock, Ross ; Dahl, Gerhard ; Dalton Dietrich, W. ; Keane, Robert. / Pyroptotic neuronal cell death mediated by the AIM2 inflammasome. In: Journal of Cerebral Blood Flow and Metabolism. 2014 ; Vol. 34, No. 4. pp. 621-629.
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