Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives

Jason P. Awe, Eric H. Gschweng, Cagustin Vega-Crespo, Jon Voutila, Mary H. Williamson, Brian Truong, Donald B. Kohn, Noriyuki Kasahara, James A. Byrne

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Autologous human induced pluripotent stemcells (hiPSCs) should allow cellular therapeuticswithout an associated immune response. This concept has been controversial since the original report that syngeneicmouse iPSCs elicited an immune response after transplantation. However, an investigative analysis of any potential acute immune responses in hiPSCs and their derivatives has yet to be conducted. In the present study, we used correlative gene expression analysis of two putative mouse “immunogenicity” genes, ZG16 and HORMAD1, to assay their human homologous expression levels in human pluripotent stem cells and their derivatives. We found that ZG16 expression is heterogeneous acrossmultiple human embryonic stem cell and hiPSC-derived cell types. Additionally, ectopic expression of ZG16 in antigen-presenting cells is insufficient to trigger a detectable response in a peripheral blood mononuclear cell coculture assay. Neither of the previous immunogenicity-associated genes in the mouse currently appears to be relevant in a human context.

Original languageEnglish (US)
Pages (from-to)136-145
Number of pages10
JournalStem Cells Translational Medicine
Volume4
Issue number2
DOIs
StatePublished - Jan 1 2015

Keywords

  • HORMAD1
  • Human embryonic stem cell
  • Human induced pluripotent stem cell
  • Immunogenicity
  • Peripheral blood mononuclear cells
  • ZG16

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives'. Together they form a unique fingerprint.

  • Cite this

    Awe, J. P., Gschweng, E. H., Vega-Crespo, C., Voutila, J., Williamson, M. H., Truong, B., Kohn, D. B., Kasahara, N., & Byrne, J. A. (2015). Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives. Stem Cells Translational Medicine, 4(2), 136-145. https://doi.org/10.5966/sctm.2014-0117