Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives

Jason P. Awe, Eric H. Gschweng, Cagustin Vega-Crespo, Jon Voutila, Mary H. Williamson, Brian Truong, Donald B. Kohn, Noriyuki Kasahara, James A. Byrne

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Autologous human induced pluripotent stemcells (hiPSCs) should allow cellular therapeuticswithout an associated immune response. This concept has been controversial since the original report that syngeneicmouse iPSCs elicited an immune response after transplantation. However, an investigative analysis of any potential acute immune responses in hiPSCs and their derivatives has yet to be conducted. In the present study, we used correlative gene expression analysis of two putative mouse “immunogenicity” genes, ZG16 and HORMAD1, to assay their human homologous expression levels in human pluripotent stem cells and their derivatives. We found that ZG16 expression is heterogeneous acrossmultiple human embryonic stem cell and hiPSC-derived cell types. Additionally, ectopic expression of ZG16 in antigen-presenting cells is insufficient to trigger a detectable response in a peripheral blood mononuclear cell coculture assay. Neither of the previous immunogenicity-associated genes in the mouse currently appears to be relevant in a human context.

Original languageEnglish (US)
Pages (from-to)136-145
Number of pages10
JournalStem cells translational medicine
Volume4
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Antigen-Presenting Cells
Coculture Techniques
Genes
Blood Cells
Transplantation
Gene Expression

Keywords

  • HORMAD1
  • Human embryonic stem cell
  • Human induced pluripotent stem cell
  • Immunogenicity
  • Peripheral blood mononuclear cells
  • ZG16

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Awe, J. P., Gschweng, E. H., Vega-Crespo, C., Voutila, J., Williamson, M. H., Truong, B., ... Byrne, J. A. (2015). Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives. Stem cells translational medicine, 4(2), 136-145. https://doi.org/10.5966/sctm.2014-0117

Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives. / Awe, Jason P.; Gschweng, Eric H.; Vega-Crespo, Cagustin; Voutila, Jon; Williamson, Mary H.; Truong, Brian; Kohn, Donald B.; Kasahara, Noriyuki; Byrne, James A.

In: Stem cells translational medicine, Vol. 4, No. 2, 01.01.2015, p. 136-145.

Research output: Contribution to journalArticle

Awe, JP, Gschweng, EH, Vega-Crespo, C, Voutila, J, Williamson, MH, Truong, B, Kohn, DB, Kasahara, N & Byrne, JA 2015, 'Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives', Stem cells translational medicine, vol. 4, no. 2, pp. 136-145. https://doi.org/10.5966/sctm.2014-0117
Awe, Jason P. ; Gschweng, Eric H. ; Vega-Crespo, Cagustin ; Voutila, Jon ; Williamson, Mary H. ; Truong, Brian ; Kohn, Donald B. ; Kasahara, Noriyuki ; Byrne, James A. / Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives. In: Stem cells translational medicine. 2015 ; Vol. 4, No. 2. pp. 136-145.
@article{538f153c01bc41148bbab2d77572e2b8,
title = "Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives",
abstract = "Autologous human induced pluripotent stemcells (hiPSCs) should allow cellular therapeuticswithout an associated immune response. This concept has been controversial since the original report that syngeneicmouse iPSCs elicited an immune response after transplantation. However, an investigative analysis of any potential acute immune responses in hiPSCs and their derivatives has yet to be conducted. In the present study, we used correlative gene expression analysis of two putative mouse “immunogenicity” genes, ZG16 and HORMAD1, to assay their human homologous expression levels in human pluripotent stem cells and their derivatives. We found that ZG16 expression is heterogeneous acrossmultiple human embryonic stem cell and hiPSC-derived cell types. Additionally, ectopic expression of ZG16 in antigen-presenting cells is insufficient to trigger a detectable response in a peripheral blood mononuclear cell coculture assay. Neither of the previous immunogenicity-associated genes in the mouse currently appears to be relevant in a human context.",
keywords = "HORMAD1, Human embryonic stem cell, Human induced pluripotent stem cell, Immunogenicity, Peripheral blood mononuclear cells, ZG16",
author = "Awe, {Jason P.} and Gschweng, {Eric H.} and Cagustin Vega-Crespo and Jon Voutila and Williamson, {Mary H.} and Brian Truong and Kohn, {Donald B.} and Noriyuki Kasahara and Byrne, {James A.}",
year = "2015",
month = "1",
day = "1",
doi = "10.5966/sctm.2014-0117",
language = "English (US)",
volume = "4",
pages = "136--145",
journal = "Stem cells translational medicine",
issn = "2157-6564",
publisher = "AlphaMed Press",
number = "2",

}

TY - JOUR

T1 - Putative immunogenicity expression profiling using human pluripotent stem cells and derivatives

AU - Awe, Jason P.

AU - Gschweng, Eric H.

AU - Vega-Crespo, Cagustin

AU - Voutila, Jon

AU - Williamson, Mary H.

AU - Truong, Brian

AU - Kohn, Donald B.

AU - Kasahara, Noriyuki

AU - Byrne, James A.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Autologous human induced pluripotent stemcells (hiPSCs) should allow cellular therapeuticswithout an associated immune response. This concept has been controversial since the original report that syngeneicmouse iPSCs elicited an immune response after transplantation. However, an investigative analysis of any potential acute immune responses in hiPSCs and their derivatives has yet to be conducted. In the present study, we used correlative gene expression analysis of two putative mouse “immunogenicity” genes, ZG16 and HORMAD1, to assay their human homologous expression levels in human pluripotent stem cells and their derivatives. We found that ZG16 expression is heterogeneous acrossmultiple human embryonic stem cell and hiPSC-derived cell types. Additionally, ectopic expression of ZG16 in antigen-presenting cells is insufficient to trigger a detectable response in a peripheral blood mononuclear cell coculture assay. Neither of the previous immunogenicity-associated genes in the mouse currently appears to be relevant in a human context.

AB - Autologous human induced pluripotent stemcells (hiPSCs) should allow cellular therapeuticswithout an associated immune response. This concept has been controversial since the original report that syngeneicmouse iPSCs elicited an immune response after transplantation. However, an investigative analysis of any potential acute immune responses in hiPSCs and their derivatives has yet to be conducted. In the present study, we used correlative gene expression analysis of two putative mouse “immunogenicity” genes, ZG16 and HORMAD1, to assay their human homologous expression levels in human pluripotent stem cells and their derivatives. We found that ZG16 expression is heterogeneous acrossmultiple human embryonic stem cell and hiPSC-derived cell types. Additionally, ectopic expression of ZG16 in antigen-presenting cells is insufficient to trigger a detectable response in a peripheral blood mononuclear cell coculture assay. Neither of the previous immunogenicity-associated genes in the mouse currently appears to be relevant in a human context.

KW - HORMAD1

KW - Human embryonic stem cell

KW - Human induced pluripotent stem cell

KW - Immunogenicity

KW - Peripheral blood mononuclear cells

KW - ZG16

UR - http://www.scopus.com/inward/record.url?scp=84921772741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84921772741&partnerID=8YFLogxK

U2 - 10.5966/sctm.2014-0117

DO - 10.5966/sctm.2014-0117

M3 - Article

C2 - 25575527

AN - SCOPUS:84921772741

VL - 4

SP - 136

EP - 145

JO - Stem cells translational medicine

JF - Stem cells translational medicine

SN - 2157-6564

IS - 2

ER -