Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth

John Bixby, Ren Zhang

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

N-cadherin is the predominant mediator of calcium-dependent adhesion in the nervous system (Takeichi, M. 1988. Development (Camb.). 102: 639-655). Investigations using antibodies to block N-cadherin function (Bixby, J. L., R. L. Pratt, J. Lilien, and L. F. Reichardt. 1987. Proc. Natl. Acad. Sci. USA. 84:2555-2569; Bixby, J. L., J. Lilien, and L. F. Reichardt. 1988. J. Cell Biol. 107:353-362; Tomaselli, K. J., K. N. Neugebauer, J. L. Bixby, J. Lilien, and L. F. Reichardt. 1988. Neuron. 1:33-43) or transfection of the N-cadherin gene into heterologous cell lines (Matsunaga, M., K. Hatta, A. Nagafuchi, and M. Takeichi. 1988. Nature (Lond.). 334:62-64) have provided evidence that N-cadherin, alone or in combination with other molecules, can participate in the induction of neurite extension. We have developed an affinity purification procedure for the isolation of whole N-cadherin from chick brain and have used the isolated protein as a substrate for neurite outgrowth. N-cadherin promotes the rapid extension of neurites from chick ciliary ganglion neurons, which extend few or no neurites on adhesive but noninducing substrates such as polylysine, tissue culture plastic, and collagens. N-cadherin is extremely potent, more so than the L1 adhesion molecule, and comparable to the extracellular matrix protein laminin. Compared to laminin, however, N-cadherin promotes outgrowth from ciliary ganglion neurons extremely rapidly and with a distinct morphology. These results provide a direct demonstration that N-cadherin is sufficient to induce neurite outgrowth when substrate bound and suggest that the mechanism(s) involved may differ from that induced by laminin.

Original languageEnglish
Pages (from-to)1253-1260
Number of pages8
JournalJournal of Cell Biology
Volume110
Issue number4
StatePublished - Apr 1 1990

Fingerprint

Cadherins
Laminin
Neurites
Parasympathetic Ganglia
Neurons
Neuronal Outgrowth
Polylysine
Extracellular Matrix Proteins
Adhesives
Nervous System
Plastics
Transfection
Collagen
Calcium
Cell Line
Antibodies
Brain

ASJC Scopus subject areas

  • Cell Biology

Cite this

Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth. / Bixby, John; Zhang, Ren.

In: Journal of Cell Biology, Vol. 110, No. 4, 01.04.1990, p. 1253-1260.

Research output: Contribution to journalArticle

@article{826c150820a84ed5becea9600c296314,
title = "Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth",
abstract = "N-cadherin is the predominant mediator of calcium-dependent adhesion in the nervous system (Takeichi, M. 1988. Development (Camb.). 102: 639-655). Investigations using antibodies to block N-cadherin function (Bixby, J. L., R. L. Pratt, J. Lilien, and L. F. Reichardt. 1987. Proc. Natl. Acad. Sci. USA. 84:2555-2569; Bixby, J. L., J. Lilien, and L. F. Reichardt. 1988. J. Cell Biol. 107:353-362; Tomaselli, K. J., K. N. Neugebauer, J. L. Bixby, J. Lilien, and L. F. Reichardt. 1988. Neuron. 1:33-43) or transfection of the N-cadherin gene into heterologous cell lines (Matsunaga, M., K. Hatta, A. Nagafuchi, and M. Takeichi. 1988. Nature (Lond.). 334:62-64) have provided evidence that N-cadherin, alone or in combination with other molecules, can participate in the induction of neurite extension. We have developed an affinity purification procedure for the isolation of whole N-cadherin from chick brain and have used the isolated protein as a substrate for neurite outgrowth. N-cadherin promotes the rapid extension of neurites from chick ciliary ganglion neurons, which extend few or no neurites on adhesive but noninducing substrates such as polylysine, tissue culture plastic, and collagens. N-cadherin is extremely potent, more so than the L1 adhesion molecule, and comparable to the extracellular matrix protein laminin. Compared to laminin, however, N-cadherin promotes outgrowth from ciliary ganglion neurons extremely rapidly and with a distinct morphology. These results provide a direct demonstration that N-cadherin is sufficient to induce neurite outgrowth when substrate bound and suggest that the mechanism(s) involved may differ from that induced by laminin.",
author = "John Bixby and Ren Zhang",
year = "1990",
month = "4",
day = "1",
language = "English",
volume = "110",
pages = "1253--1260",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

TY - JOUR

T1 - Purified N-cadherin is a potent substrate for the rapid induction of neurite outgrowth

AU - Bixby, John

AU - Zhang, Ren

PY - 1990/4/1

Y1 - 1990/4/1

N2 - N-cadherin is the predominant mediator of calcium-dependent adhesion in the nervous system (Takeichi, M. 1988. Development (Camb.). 102: 639-655). Investigations using antibodies to block N-cadherin function (Bixby, J. L., R. L. Pratt, J. Lilien, and L. F. Reichardt. 1987. Proc. Natl. Acad. Sci. USA. 84:2555-2569; Bixby, J. L., J. Lilien, and L. F. Reichardt. 1988. J. Cell Biol. 107:353-362; Tomaselli, K. J., K. N. Neugebauer, J. L. Bixby, J. Lilien, and L. F. Reichardt. 1988. Neuron. 1:33-43) or transfection of the N-cadherin gene into heterologous cell lines (Matsunaga, M., K. Hatta, A. Nagafuchi, and M. Takeichi. 1988. Nature (Lond.). 334:62-64) have provided evidence that N-cadherin, alone or in combination with other molecules, can participate in the induction of neurite extension. We have developed an affinity purification procedure for the isolation of whole N-cadherin from chick brain and have used the isolated protein as a substrate for neurite outgrowth. N-cadherin promotes the rapid extension of neurites from chick ciliary ganglion neurons, which extend few or no neurites on adhesive but noninducing substrates such as polylysine, tissue culture plastic, and collagens. N-cadherin is extremely potent, more so than the L1 adhesion molecule, and comparable to the extracellular matrix protein laminin. Compared to laminin, however, N-cadherin promotes outgrowth from ciliary ganglion neurons extremely rapidly and with a distinct morphology. These results provide a direct demonstration that N-cadherin is sufficient to induce neurite outgrowth when substrate bound and suggest that the mechanism(s) involved may differ from that induced by laminin.

AB - N-cadherin is the predominant mediator of calcium-dependent adhesion in the nervous system (Takeichi, M. 1988. Development (Camb.). 102: 639-655). Investigations using antibodies to block N-cadherin function (Bixby, J. L., R. L. Pratt, J. Lilien, and L. F. Reichardt. 1987. Proc. Natl. Acad. Sci. USA. 84:2555-2569; Bixby, J. L., J. Lilien, and L. F. Reichardt. 1988. J. Cell Biol. 107:353-362; Tomaselli, K. J., K. N. Neugebauer, J. L. Bixby, J. Lilien, and L. F. Reichardt. 1988. Neuron. 1:33-43) or transfection of the N-cadherin gene into heterologous cell lines (Matsunaga, M., K. Hatta, A. Nagafuchi, and M. Takeichi. 1988. Nature (Lond.). 334:62-64) have provided evidence that N-cadherin, alone or in combination with other molecules, can participate in the induction of neurite extension. We have developed an affinity purification procedure for the isolation of whole N-cadherin from chick brain and have used the isolated protein as a substrate for neurite outgrowth. N-cadherin promotes the rapid extension of neurites from chick ciliary ganglion neurons, which extend few or no neurites on adhesive but noninducing substrates such as polylysine, tissue culture plastic, and collagens. N-cadherin is extremely potent, more so than the L1 adhesion molecule, and comparable to the extracellular matrix protein laminin. Compared to laminin, however, N-cadherin promotes outgrowth from ciliary ganglion neurons extremely rapidly and with a distinct morphology. These results provide a direct demonstration that N-cadherin is sufficient to induce neurite outgrowth when substrate bound and suggest that the mechanism(s) involved may differ from that induced by laminin.

UR - http://www.scopus.com/inward/record.url?scp=0025215306&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025215306&partnerID=8YFLogxK

M3 - Article

VL - 110

SP - 1253

EP - 1260

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -