Purified hybrid cells from dendritic cell and tumor cell fusions are superior activators of antitumor immunity

Mingjiang Xu, Runxiang Zhao, Xingwei Sui, Fengping Xu, Zhizhuang Joe Zhao

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The use of fusions between dendritic cells (DCs) and tumor cells as vaccines has been proved very effective in stimulating antitumor immune responses, both in animal studies and in early human clinical trials. Because of the difficulty of purifying the hybrid cells from the fusion, fusion mixtures were used in these studies. Recently, we developed a technique using fluorescent-dye staining and fluorescence-activated cell sorting that enabled the hybrid cells to be instantly purified from the fusion mixture [10]. In the present study, the hybrid cells were purified from a fusion between mouse DCs and B16F0 melanoma tumor cells using the new technique. The purified cells, named instant dendritomas (IDs) were then compared with fusion mixtures in stimulating antitumor immune responses. The results from cytotoxicity assays, interferon-γ production and in vivo lung tumor metastasis demonstrated that IDs are more effective than fusion mixture in stimulating antitumor immunity. Meanwhile, there was no significant difference in the antitumor immunities activated by IDs from allogenic fusion or IDs from syngenic fusion.

Original languageEnglish (US)
Pages (from-to)456-462
Number of pages7
JournalCancer Immunology, Immunotherapy
Volume50
Issue number9
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Cell Fusion
Hybrid Cells
Dendritic Cells
Immunity
Neoplasms
Fluorescent Dyes
Interferons
Melanoma
Flow Cytometry
Vaccines
Clinical Trials
Staining and Labeling
Neoplasm Metastasis
Lung

Keywords

  • Dendritic cell
  • Fusion
  • Instant dendritoma
  • Tumor
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cite this

Purified hybrid cells from dendritic cell and tumor cell fusions are superior activators of antitumor immunity. / Xu, Mingjiang; Zhao, Runxiang; Sui, Xingwei; Xu, Fengping; Zhao, Zhizhuang Joe.

In: Cancer Immunology, Immunotherapy, Vol. 50, No. 9, 2001, p. 456-462.

Research output: Contribution to journalArticle

Xu, Mingjiang ; Zhao, Runxiang ; Sui, Xingwei ; Xu, Fengping ; Zhao, Zhizhuang Joe. / Purified hybrid cells from dendritic cell and tumor cell fusions are superior activators of antitumor immunity. In: Cancer Immunology, Immunotherapy. 2001 ; Vol. 50, No. 9. pp. 456-462.
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