Abstract
SV-40 DNA sheared form was coupled in a stable covalent bond to cyanogen bromide activated Sepharose. Under the conditions used at least 80% of the DNA was bound to Sepharose. The T1/2 of hybridization of 0.5 μg/ml of SV-40 cRNA to SV-40 DNA-Sepharose was 1 hr. This rate of hybridization is sufficiently rapid to purify SV-40 sequences from solutions containing as little as 0.05-0.1 μg/ml. Nonspecific hybridization of RNA is in the range of 0.1-0.2% of the total input RNA. The DNA-Sepharose is fairly stable and can be reused several times to purify RNA. The SV-40 DNA-Sepharose was used to select large quantities of virus specific RNA from SV-40 infected BS-C-1 cells. The virus specific RNA when added to cell-free extracts from wheat germ was shown to direct the synthesis of the major viral structural protein VP-1.
Original language | English (US) |
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Pages (from-to) | 4215-4220 |
Number of pages | 6 |
Journal | Biochemistry |
Volume | 14 |
Issue number | 19 |
DOIs | |
State | Published - Sep 1 1975 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry