The methods of molecular sieving through gel filtration on Sephadex G-50 (fine grade) and ion exchange chromatography on TEAE and DEAE cellulose and CMC were used for the purification of pancreozymin from concentrates of hog small intestine. A purification of at least 30-fold over the starting material could be obtained in two steps. From the migration of biologic activity under high voltage paper electrophoresis, the isoelectrical point of pancreozymin was estimated to be around pH 8. The tentative molecular weight of pancreozymin as determined by elution volume (Rf values) by comparison with known polypeptides is 4500 to 5000.
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