Purification of Pancreozymin

A. P.S. Dhariwal, A. V. Schally, J. Meyer, D. C.H. Sun, J. E. Jorpes, V. Mutt

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The methods of molecular sieving through gel filtration on Sephadex G-50 (fine grade) and ion exchange chromatography on TEAE and DEAE cellulose and CMC were used for the purification of pancreozymin from concentrates of hog small intestine. A purification of at least 30-fold over the starting material could be obtained in two steps. From the migration of biologic activity under high voltage paper electrophoresis, the isoelectrical point of pancreozymin was estimated to be around pH 8. The tentative molecular weight of pancreozymin as determined by elution volume (Rf values) by comparison with known polypeptides is 4500 to 5000.

Original languageEnglish (US)
Pages (from-to)316-321
Number of pages6
JournalGastroenterology
Volume44
Issue number3
DOIs
StatePublished - Jan 1 1963
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Purification of Pancreozymin'. Together they form a unique fingerprint.

  • Cite this

    Dhariwal, A. P. S., Schally, A. V., Meyer, J., Sun, D. C. H., Jorpes, J. E., & Mutt, V. (1963). Purification of Pancreozymin. Gastroenterology, 44(3), 316-321. https://doi.org/10.1016/S0016-5085(63)80142-0