Purification of human monocyte-macrophages by a cell suspension culture technique

Henry C. Stevenson, Paul Katz, Anthony S. Fauci

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Human monocytes transform after 6 days of culture into large monocyte-macrophages without adherence. They are then easily separated from other types of mononuclear cells on a Hypaque-Ficoll gradient. These cells can be obtained in suspension at greater than 90% purity. The method is simple, highly reproducible, and does not require complex fractionation equipment. This method can be utilized to obtain large numbers of highly purified viable monocytes without introducing the potential artifacts resulting from adherence of cells to surfaces and subsequent dislodgement procedures.

Original languageEnglish (US)
Pages (from-to)94-103
Number of pages10
JournalCellular Immunology
Volume53
Issue number1
DOIs
StatePublished - Jan 1 1980

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ASJC Scopus subject areas

  • Immunology

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