TY - JOUR
T1 - Purification of galactofipids by high-performance liquid chromatography for monolayer and Langmuir-Blodgett film studies
AU - Gallant, Judith
AU - Leblanc, Roger M.
N1 - Funding Information:
The assistanceo f Drs. G. Picard and B. Zelent is greatly appreciatedS. pecial thanksa re due to Dr. F. Bellemarea nd to Mr. G. Munger for their preliminaryw ork on chloroplastl ipidspurified by liquid chromatographyT. his work was supportedb y grants from the Natural Sciencesa nd Engineering Research Council of Canada (NSERC) and the Fonds pour la Formation de Chercheurse t 1’Aided la Recherche (FCAR). Financial support as postgraduates cholarshipsc amef rom FCAR to J.G.
PY - 1991
Y1 - 1991
N2 - In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
AB - In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
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U2 - 10.1016/S0021-9673(01)88769-0
DO - 10.1016/S0021-9673(01)88769-0
M3 - Article
AN - SCOPUS:0026081079
VL - 542
SP - 307
EP - 316
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -