Abstract
In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
| Original language | English |
|---|---|
| Pages (from-to) | 307-316 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 542 |
| Issue number | C |
| DOIs | |
| State | Published - Jan 1 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine