Purification of galactofipids by high-performance liquid chromatography for monolayer and Langmuir-Blodgett film studies

Judith Gallant, Roger Leblanc

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.

Original languageEnglish
Pages (from-to)307-316
Number of pages10
JournalJournal of Chromatography A
Volume542
Issue numberC
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Galactolipids
Langmuir Blodgett films
High performance liquid chromatography
Purification
Monolayers
Nitrogen
Fluorescence
High Pressure Liquid Chromatography
Water
Liquid chromatography
Liquid Chromatography
Isotherms
Lipids
Pressure
chlorophyll a

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Purification of galactofipids by high-performance liquid chromatography for monolayer and Langmuir-Blodgett film studies. / Gallant, Judith; Leblanc, Roger.

In: Journal of Chromatography A, Vol. 542, No. C, 01.01.1991, p. 307-316.

Research output: Contribution to journalArticle

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