Abstract
In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
| Original language | English |
|---|---|
| Pages (from-to) | 307-316 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 542 |
| Issue number | C |
| DOIs | |
| State | Published - Jan 1 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine
Cite this
Purification of galactofipids by high-performance liquid chromatography for monolayer and Langmuir-Blodgett film studies. / Gallant, Judith; Leblanc, Roger.
In: Journal of Chromatography A, Vol. 542, No. C, 01.01.1991, p. 307-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification of galactofipids by high-performance liquid chromatography for monolayer and Langmuir-Blodgett film studies
AU - Gallant, Judith
AU - Leblanc, Roger
PY - 1991/1/1
Y1 - 1991/1/1
N2 - In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
AB - In order to obtain pure galactolipids usable for monolayer work at the nitrogen-water interface, and also for fluorescence intensity measurements in Langmuir-Blodgett films when mixed with chlorophyll a, purification procedures for mono- and digalactosyldiacylglycerol were established using high-performance liquid chromatography. The rapid and efficient methods described were applied to commercial samples and enriched extracted fractions of lipids obtained by preparative liquid chromatography. Surface pressure-area isotherms at the nitrogen-water interface of purified and unpurified samples are also reported. The fluorescence spectra of chlorophyll a mixed with purified and unpurified galactolipids in Langmuir-Blodgett films clearly show the necessity for the purification.
UR - http://www.scopus.com/inward/record.url?scp=0026081079&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026081079&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)88769-0
DO - 10.1016/S0021-9673(01)88769-0
M3 - Article
AN - SCOPUS:0026081079
VL - 542
SP - 307
EP - 316
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - C
ER -