Purification of Actin from Cardiac Muscle

Henry G. Zot, James D. Potter

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Actin from cardiac acetone and ether powders is compared to actin from skeletal acetone powder using a modification of an established extraction procedure. The yield of actin from cardiac ether powder is nearly the same as the yield from skeletal acetone powder whereas significantly less actin is obtained from cardiac acetone powder. Sodium dodecyl sulfate polyacrylamide gel electrophoresis shows the actins from each of the sources to be virtually identical in terms of purity and mobility. Molecular sieve chromatography of G-actin demonstrates cardiac actin from ether powder to have identical polymerization and mobility properties as skeletal actin from acetone powder. A simplified procedure, developed for highly purified actin from muscle powder and prepared in a single day is presented. The storage of F-actin at -80°C is also discussed.

Original languageEnglish (US)
Pages (from-to)381-395
Number of pages15
JournalPreparative Biochemistry
Volume11
Issue number4
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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