Purification of a colony-stimulating factor from cultured pancreatic carcinoma cells

M. Wu, J. K. Cini, A. A. Yunis

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Serum-free conditioned medium prepared from an established line of human pancreatic carcinoma (MIA PaCa-2) provides a rich source of colony-stimulating factor (CSF). Two activities distinctly separable by isoelectrofocusing have been identified: a high molecular weight CSF exhibiting greater activity in mouse bone marrow and a low molecular weight CSF more active in human bone marrow. The high molecular weight CSF has been purified 1000-fold to apparent homogeneity by a two-step procedure incuding isoelectrofocusing and gel filtration chromatography. The purified CSF has a molecular weight of 50,000 an an isoelectric point of 3.7 to 4.6. It is a glycoprotein as shown by periodic acid-Schiff stain and exhibits greater activity in mouse marrow than in human marrow.

Original languageEnglish (US)
Pages (from-to)6226-6228
Number of pages3
JournalJournal of Biological Chemistry
Volume254
Issue number14
StatePublished - Dec 1 1979

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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