Abstract
Serum-free conditioned medium prepared from an established line of human pancreatic carcinoma (MIA PaCa-2) provides a rich source of colony-stimulating factor (CSF). Two activities distinctly separable by isoelectrofocusing have been identified: a high molecular weight CSF exhibiting greater activity in mouse bone marrow and a low molecular weight CSF more active in human bone marrow. The high molecular weight CSF has been purified 1000-fold to apparent homogeneity by a two-step procedure incuding isoelectrofocusing and gel filtration chromatography. The purified CSF has a molecular weight of 50,000 an an isoelectric point of 3.7 to 4.6. It is a glycoprotein as shown by periodic acid-Schiff stain and exhibits greater activity in mouse marrow than in human marrow.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6226-6228 |
| Number of pages | 3 |
| Journal | Journal of Biological Chemistry |
| Volume | 254 |
| Issue number | 14 |
| State | Published - 1979 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology