Abstract
RluD is the pseudouridine (ψ) synthase responsible for forming ψ1911, ψ1915 and ψ1917 in Escherichia coli 23S RNA. Out of the 11 ψ synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of ψ synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 Å and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 Å. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 Å resolution.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1871-1873 |
| Number of pages | 3 |
| Journal | Acta Crystallographica - Section D Biological Crystallography |
| Volume | 59 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2003 |
ASJC Scopus subject areas
- Structural Biology
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Del Campo, M., Ofengand, J., & Malhotra, A. (2003). Purification and crystallization of Escherichia coli pseudouridine synthase RluD. Acta Crystallographica - Section D Biological Crystallography, 59(10), 1871-1873. https://doi.org/10.1107/S0907444903018468