Purification and crystallization of Escherichia coli pseudouridine synthase RluD

Mark Del Campo, James Ofengand, Arun Malhotra

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

RluD is the pseudouridine (ψ) synthase responsible for forming ψ1911, ψ1915 and ψ1917 in Escherichia coli 23S RNA. Out of the 11 ψ synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of ψ synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 Å and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 Å. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 Å resolution.

Original languageEnglish (US)
Pages (from-to)1871-1873
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number10
DOIs
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Structural Biology

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