TY - JOUR
T1 - Purification and crystallization of Escherichia coli pseudouridine synthase RluD
AU - Del Campo, Mark
AU - Ofengand, James
AU - Malhotra, Arun
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - RluD is the pseudouridine (ψ) synthase responsible for forming ψ1911, ψ1915 and ψ1917 in Escherichia coli 23S RNA. Out of the 11 ψ synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of ψ synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 Å and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 Å. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 Å resolution.
AB - RluD is the pseudouridine (ψ) synthase responsible for forming ψ1911, ψ1915 and ψ1917 in Escherichia coli 23S RNA. Out of the 11 ψ synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of ψ synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 Å and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 Å. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 Å resolution.
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U2 - 10.1107/S0907444903018468
DO - 10.1107/S0907444903018468
M3 - Article
C2 - 14501142
AN - SCOPUS:0141925457
VL - 59
SP - 1871
EP - 1873
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 10
ER -