Purification and crystallization of Escherichia coli pseudouridine synthase RluD

Mark Del Campo, James Ofengand, Arun Malhotra

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

RluD is the pseudouridine (ψ) synthase responsible for forming ψ1911, ψ1915 and ψ1917 in Escherichia coli 23S RNA. Out of the 11 ψ synthases in E. coli, only cells lacking RluD show a severe growth defect. In addition, RluD belongs to the RluA family of ψ synthases, one of the two remaining families without a representative crystal structure. In this paper, the crystallization of selenomethionine-substituted RluD by the hanging-drop method is reported. The crystals diffract to 1.9 Å and belong to space group P43212, with unit-cell parameters a = b = 75.14, c = 181.81 Å. Synchrotron radiation was used on a single crystal to collect a complete multiwavelength anomalous dispersion (MAD) data set to 2.0 Å resolution.

Original languageEnglish
Pages (from-to)1871-1873
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number10
DOIs
StatePublished - Oct 1 2003

Fingerprint

Escherichia
Crystallization
purification
Escherichia coli
Purification
crystallization
Selenomethionine
Synchrotrons
Synchrotron radiation
cells
synchrotron radiation
Crystal structure
Single crystals
RNA
Radiation
Defects
Crystals
crystal structure
single crystals
defects

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Purification and crystallization of Escherichia coli pseudouridine synthase RluD. / Del Campo, Mark; Ofengand, James; Malhotra, Arun.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 10, 01.10.2003, p. 1871-1873.

Research output: Contribution to journalArticle

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