Purification and crystallization of Bacillus subtilis NrnA, a novel enzyme involved in nanoRNA degradation

Claudiu M. Nelersa, Brad J. Schmier, Arun Malhotra

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The final step in RNA degradation is the hydrolysis of RNA fragments five nucleotides or less in length (nanoRNA) to mononucleotides. In Escherichia coli this step is carried out by oligoribonuclease (Orn), a DEDD-family exoribonuclease that is conserved throughout eukaryotes. However, many bacteria lack Orn homologs, and an unrelated DHH-family phosphoesterase, NrnA, has recently been identified as one of the enzymes responsible for nanoRNA degradation in Bacillus subtilis. To understand its mechanism of action, B. subtilis NrnA was purified and crystallized at room temperature using the hanging-drop vapor-diffusion method with PEG 4000, PEG 3350 or PEG MME 2000 as precipitant. The crystals belonged to the primitive monoclinic space group P21, with unit-cell parameters a = 50.62, b = 121.3, c = 123.4 Å, = 90, β = 91.31, γ = 90°.

Original languageEnglish (US)
Pages (from-to)1235-1238
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number10
DOIs
StatePublished - Oct 2011

Keywords

  • Bacillus subtilis
  • exonucleases
  • pAp phosphatase
  • RNA degradation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'Purification and crystallization of Bacillus subtilis NrnA, a novel enzyme involved in nanoRNA degradation'. Together they form a unique fingerprint.

Cite this