Abstract
Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.
| Original language | English |
|---|---|
| Pages (from-to) | 209-219 |
| Number of pages | 11 |
| Journal | Acta Endocrinologica |
| Volume | 80 |
| Issue number | 2 |
| State | Published - Dec 1 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
Cite this
Purification and characterisation of two porcine hypothalamic fractions with LH releasing activity : evidence for a single LH and FSH releasing hormone. / Sandow, J.; Enzmann, F.; Arimura, A.; Redding, T. W.; Schally, Andrew V.
In: Acta Endocrinologica, Vol. 80, No. 2, 01.12.1975, p. 209-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Purification and characterisation of two porcine hypothalamic fractions with LH releasing activity
T2 - evidence for a single LH and FSH releasing hormone
AU - Sandow, J.
AU - Enzmann, F.
AU - Arimura, A.
AU - Redding, T. W.
AU - Schally, Andrew V
PY - 1975/12/1
Y1 - 1975/12/1
N2 - Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.
AB - Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016820563&partnerID=8YFLogxK
M3 - Article
VL - 80
SP - 209
EP - 219
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 2
ER -