Purification and characterisation of two porcine hypothalamic fractions with LH releasing activity: evidence for a single LH and FSH releasing hormone

J. Sandow; F. Enzmann; A. Arimura; T. W. Redding; A. V. Schally

doi:10.1530/acta.0.0800209

Purification and characterisation of two porcine hypothalamic fractions with LH releasing activity: evidence for a single LH and FSH releasing hormone

J. Sandow, F. Enzmann, A. Arimura, T. W. Redding, A. V. Schally

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.

Original language	English (US)
Pages (from-to)	209-219
Number of pages	11
Journal	Unknown Journal
Volume	80
Issue number	2
DOIs	https://doi.org/10.1530/acta.0.0800209
State	Published - 1975
Externally published	Yes

ASJC Scopus subject areas

Endocrinology

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title = "Purification and characterisation of two porcine hypothalamic fractions with LH releasing activity: evidence for a single LH and FSH releasing hormone",

abstract = "Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.",

author = "J. Sandow and F. Enzmann and A. Arimura and Redding, {T. W.} and Schally, {A. V.}",

year = "1975",

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T2 - evidence for a single LH and FSH releasing hormone

AU - Sandow, J.

AU - Enzmann, F.

AU - Arimura, A.

AU - Redding, T. W.

AU - Schally, A. V.

PY - 1975

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AB - Two fractions with LH releasing activity were obtained from porcine hypothalami by Enzmann et al. (1972). These fractions were designated E 57 and E 203. By comparison with pure natural LH RH and synthetic LH RH, E 203 was identified as purified natural LH RH. E 57, which has only 25% of the activity of E 203, was further evaluated for its biological and immunological activity. E 57 has the same ratio of LH/FSH releasing activity as natural LH RH. The binding to a specific LH RH antiserum is identical with that of natural LH RH and E 203. It is concluded, that E 57 is an artifact of isolation derived from natural LH RH. These findings can be considered as additional evidence for the identity of the hypothalamic LH and FSH releasing hormone, since both gonadotrophins are stimulated by the two hypothalamic fractions.

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