Purification and amino acid sequence of a noncalcitonin secretory peptide derived from preprocalcitonin

R. S. Birnbaum, W. Mahoney, B. A. Roos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A previous report from this laboratory provided immunochemical and biochemical evidence for the existence of a secretory peptide derived from the noncalcitonin region of rat preprocalcitonin. By a variety of criteria, we demonstrated that this naturally occurring peptide was similar, if not identical, to a synthetic peptide which consisted of the NH2-terminal 16 residues of the calcitonin mRNA translation product. We have now purified this peptide from rat medullary thyroid carcinoma and sequenced it. A rat tumor of the 1-2-4 tumor series was extracted in 0.1 N HCl yielding 900 μg of immunoreactive peptide. The peptide was purified to homogeneity by: trichloroacetic acid precipitation of contaminating protein; gel filtration; and finally, reverse phase high pressure liquid chromatography. Overall yield was approximately 24%. Amino acid analysis and sequencing of the peptide yielded a composition and sequence identical with that of the synthetic peptide.

Original languageEnglish
Pages (from-to)5463-5466
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number9
StatePublished - Jan 1 1983

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Purification
Amino Acid Sequence
Amino Acids
Peptides
Rats
Tumors
High pressure liquid chromatography
Trichloroacetic Acid
preprocalcitonin
Protein Sequence Analysis
Calcitonin
Protein Biosynthesis
Reverse-Phase Chromatography
Gel Chromatography
Neoplasms
Gels
High Pressure Liquid Chromatography
Messenger RNA
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Purification and amino acid sequence of a noncalcitonin secretory peptide derived from preprocalcitonin. / Birnbaum, R. S.; Mahoney, W.; Roos, B. A.

In: Journal of Biological Chemistry, Vol. 258, No. 9, 01.01.1983, p. 5463-5466.

Research output: Contribution to journalArticle

Birnbaum, R. S. ; Mahoney, W. ; Roos, B. A. / Purification and amino acid sequence of a noncalcitonin secretory peptide derived from preprocalcitonin. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 9. pp. 5463-5466.
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