Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores

Brian Marples, L. Downing, K. E. Sawarynski, J. N. Finkelstein, J. P. Williams, A. A. Martinez, G. D. Wilson, M. D. Sims

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Exposure to infectious microbes is a likely confounder after a nuclear terrorism event. In combination with radiation, morbidity and mortality from an infection may increase significantly. Pulmonary damage after low-dose low-LET irradiation is characterized by an initial diffuse alveolar inflammation. By contrast, inhaled fungal spores produce localized damage around pulmonary bronchioles. In the present study, we assessed lung injury in C57BL/6 mice after combined exposures to whole-body X radiation and inhaled fungal spores. Either animals were exposed to Aspergillus spores and immediately irradiated with 2 Gy, or the inoculation and irradiation were separated by 8 weeks. Pulmonary injury was assessed at 24 and 48 h and 1, 2, 4, 8, and 24 weeks later using standard H&E-stained sections and compared with sham-treated age-matched controls. Immunohistochemistry for invasive inflammatory cells (macrophages, neutrophils and B and T lymphocytes) was performed. A semi-quantitative assessment of pulmonary injury was made using three distinct parameters: local infiltration of inflammatory cells, diffuse inflammation, and thickening and distortion of alveolar architecture. Radiation-induced changes in lung architecture were most evident during the first 2 weeks postexposure. Fungal changes were seen over the first 4 weeks. Simultaneous combined exposures significantly increased the duration of acute pulmonary damage up to 24 weeks (P < 0.01). In contrast, administration of the fungus 8 weeks after irradiation did not produce enhanced levels of acute pulmonary damage. These data imply that the inhalation of fungal spores at the time of a radiation exposure alters the susceptibility of the lungs to radiation-induced injury.

Original languageEnglish (US)
Pages (from-to)501-509
Number of pages9
JournalRadiation Research
Volume175
Issue number4
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

Fingerprint

Linear Energy Transfer
spores
Fungal Spores
Lung Injury
lungs
Radiation
damage
dosage
Lung
radiation
irradiation
terrorism
neutrophils
Aspergillus
macrophages
fungi
mortality
lymphocytes
respiration
radiation dosage

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Marples, B., Downing, L., Sawarynski, K. E., Finkelstein, J. N., Williams, J. P., Martinez, A. A., ... Sims, M. D. (2011). Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores. Radiation Research, 175(4), 501-509. https://doi.org/10.1667/RR2379.1

Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores. / Marples, Brian; Downing, L.; Sawarynski, K. E.; Finkelstein, J. N.; Williams, J. P.; Martinez, A. A.; Wilson, G. D.; Sims, M. D.

In: Radiation Research, Vol. 175, No. 4, 01.04.2011, p. 501-509.

Research output: Contribution to journalArticle

Marples, B, Downing, L, Sawarynski, KE, Finkelstein, JN, Williams, JP, Martinez, AA, Wilson, GD & Sims, MD 2011, 'Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores', Radiation Research, vol. 175, no. 4, pp. 501-509. https://doi.org/10.1667/RR2379.1
Marples B, Downing L, Sawarynski KE, Finkelstein JN, Williams JP, Martinez AA et al. Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores. Radiation Research. 2011 Apr 1;175(4):501-509. https://doi.org/10.1667/RR2379.1
Marples, Brian ; Downing, L. ; Sawarynski, K. E. ; Finkelstein, J. N. ; Williams, J. P. ; Martinez, A. A. ; Wilson, G. D. ; Sims, M. D. / Pulmonary injury after combined exposures to low-dose low-LET radiation and fungal spores. In: Radiation Research. 2011 ; Vol. 175, No. 4. pp. 501-509.
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