Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice

S. Kaushal, V. F. La Russa, E. R. Hall, S. Gartner, J. H. Kim, L. P. Perera, Z. Yu, S. W. Kessler, J. D. Mosca

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1 Scopus citations

Abstract

In order to develop a convenient small-animal model that can support the differentiation of human bone-marrow-derived CD34+ cells, we transplanted SCID mice with an immortalized human stromal cell line, Lof(11-10). The Lof(11-10) cell line has been characterized to produce human cytokines capable of supporting primitive human hematopoietic cell proliferation in vitro. Intraperitoneal injection of Lof(11-10) cells into irradiated SCID mice by itself resulted in a dose-dependent survival of the mice from lethal irradiation. The radioprotective survival was reflected by an increase in the growth and number of mouse bone-marrow-derived committed hematopoietic progenitors. The Lof(11-10) cells localized to the spleen, but not to the bone marrow of these animals and resulted in detectable levels of circulating human IL-6 in their plasma. Secondary intravenous injections of either human or simian CD34+ cells into the Lof(11-10)-transplanted SCID mice resulted in engraftment of injected cells within the bone marrow of these mice. The utility of this small-animal model that allows the growth and differentiation of human CD34+ cells and its potential use in clinical gene therapy protocols are discussed.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalJournal of Biomedical Science
Volume4
Issue number2-3
DOIs
StatePublished - Mar 1997

Keywords

  • CD34+ cells
  • Hematopoiesis
  • Microenvironment
  • SCID mice
  • Stromal cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Kaushal, S., La Russa, V. F., Hall, E. R., Gartner, S., Kim, J. H., Perera, L. P., Yu, Z., Kessler, S. W., & Mosca, J. D. (1997). Providing a microenvironment for the development of human CD34+ hematopoietic cells in SCID mice. Journal of Biomedical Science, 4(2-3), 61-68. https://doi.org/10.1007/BF02255595