Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems

Pierre Gilles Henry, Malgorzata Marjanska, Jamie D. Walls, Julien Valette, Rolf Gruetter, Kâmil Uǧurbil

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Proton-observed carbon-edited (POCE) NMR spectroscopy is commonly used to measure 13C labeling with higher sensitivity compared to direct 13C NMR spectroscopy, at the expense of spectral resolution. For weakly coupled first-order spin systems, the multiplet signal at a specific proton chemical shift in POCE spectra directly reflects 13C enrichment of the carbon attached to this proton. The present study demonstrates that this is not necessarily the case for strongly coupled second-order spin systems. In such cases NMR signals can be detected in the POCE spectra even at chemical shifts corresponding to protons bound to 12C. This effect is demonstrated theoretically with density matrix calculations and simulations, and experimentally with measured POCE spectra of [3-13C] glutamate.

Original languageEnglish (US)
Pages (from-to)250-257
Number of pages8
JournalMagnetic Resonance in Medicine
Volume55
Issue number2
DOIs
StatePublished - Feb 1 2006
Externally publishedYes

Keywords

  • C editing
  • Density matrix simulation
  • Glutamate
  • POCE NMR spectroscopy
  • Strong coupling

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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