Proton currents through amiloride-sensitive Na+ channels in isolated hamster taste cells: Enhancement by vasopressin and CAMP

Timothy A. Gilbertson, Stephen D. Roper, Sue C. Kinnamon

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

Amiloride has been suggested to inhibit responses to a variety of taste stimuli, including salty, sweet, and sour (acid). To test for the involvement of amiloride-sensitive Na+ channels in the transduction of acid stimuli, fungi-form taste receptor cells were examined using patch-clamp techniques. Approximately one-half of all cells had amiloride-sensitive Na+ currents (INa) with a Ki value near 0.2 μM amiloride. After blocking voltage-gated conductances, cells having amiloride sensitivity were tested for responses to acid stimuli. Over three-fourths of cells showed an inward proton current (IH+) with an extrapolated reversal potential near approximately +150 mV, which was completely blocked by amiloride (30 μM). Treatment of isolated taste cells with arginine8-vasopressin caused equivalent increases in both INa and IH+; each effect was mimicked by 8-Br-cAMP. Taken together, these results indicate that protons permeate amiloride-sensitive Na+ channels in hamster fungiform taste cells and contribute to acid transduction.

Original languageEnglish (US)
Pages (from-to)931-942
Number of pages12
JournalNeuron
Volume10
Issue number5
DOIs
StatePublished - May 1993
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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