Proton currents through amiloride-sensitive Na+ channels in isolated hamster taste cells: Enhancement by vasopressin and CAMP

Timothy A. Gilbertson, Stephen D. Roper, Sue C. Kinnamon

Research output: Contribution to journalArticlepeer-review

133 Scopus citations


Amiloride has been suggested to inhibit responses to a variety of taste stimuli, including salty, sweet, and sour (acid). To test for the involvement of amiloride-sensitive Na+ channels in the transduction of acid stimuli, fungi-form taste receptor cells were examined using patch-clamp techniques. Approximately one-half of all cells had amiloride-sensitive Na+ currents (INa) with a Ki value near 0.2 μM amiloride. After blocking voltage-gated conductances, cells having amiloride sensitivity were tested for responses to acid stimuli. Over three-fourths of cells showed an inward proton current (IH+) with an extrapolated reversal potential near approximately +150 mV, which was completely blocked by amiloride (30 μM). Treatment of isolated taste cells with arginine8-vasopressin caused equivalent increases in both INa and IH+; each effect was mimicked by 8-Br-cAMP. Taken together, these results indicate that protons permeate amiloride-sensitive Na+ channels in hamster fungiform taste cells and contribute to acid transduction.

Original languageEnglish (US)
Pages (from-to)931-942
Number of pages12
Issue number5
StatePublished - May 1993
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Proton currents through amiloride-sensitive Na<sup>+</sup> channels in isolated hamster taste cells: Enhancement by vasopressin and CAMP'. Together they form a unique fingerprint.

Cite this