Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork

Sanjoy K Bhattacharya, Edward J. Rockwood, Scott D. Smith, Vera L. Bonilha, John S. Crabb, Rachel W. Kuchtey, Nahid G. Robertson, Neal S. Peachey, Cynthia C. Morton, John W. Crabb

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The etiology of primary open angle glaucoma, a leading cause of age-related blindness, remains poorly defined, although elevated intraocular pressure (IOP) contributes to the disease progression. To better understand the mechanisms causing elevated IOP from aqueous humor circulation, we pursued proteomic analyses of trabecular meshwork (TM) from glaucoma and age-matched control donors. These analyses demonstrated that Cochlin, a protein associated with deafness disorder DFNA9, is present in glaucomatous but absent in normal TM. Cochlin was also detected in TM from the glaucomatous DBA/2J mouse preceding elevated IOP but found to be absent in three other mouse lines that do not develop elevated IOP. Histochemical analyses revealed co-deposits of Cochlin and mucopolysaccharide in human TM around Schlemm's canal, similar to that observed in the cochlea in DFNA9 deafness. Purified Cochlin was found to aggregate after sheer stress and to induce the aggregation of TM cells in vitro. Age-dependent in vivo increases in Cochlin were observed in glaucomatous TM, concomitant with a decrease in type II collagen, suggesting that Cochlin may disrupt the TM architecture and render components like collagen more susceptible to degradation and collapse. Overall, these observations suggest that Cochlin contributes to elevated IOP in primary open angle glaucoma through altered interactions within the TM extracellular matrix, resulting in cell aggregation, mucopolysaccharide deposition, and significant obstruction of the aqueous humor circulation.

Original languageEnglish
Pages (from-to)6080-6084
Number of pages5
JournalJournal of Biological Chemistry
Volume280
Issue number7
DOIs
StatePublished - Feb 18 2005
Externally publishedYes

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Trabecular Meshwork
Proteomics
Deposits
Intraocular Pressure
Glycosaminoglycans
Agglomeration
Aqueous Humor
Deafness
Collagen Type II
Canals
Collagen
Cell Aggregation
Inbred DBA Mouse
Cochlea
Blindness
Degradation
Glaucoma
Extracellular Matrix
Disease Progression
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bhattacharya, S. K., Rockwood, E. J., Smith, S. D., Bonilha, V. L., Crabb, J. S., Kuchtey, R. W., ... Crabb, J. W. (2005). Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork. Journal of Biological Chemistry, 280(7), 6080-6084. https://doi.org/10.1074/jbc.M411233200

Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork. / Bhattacharya, Sanjoy K; Rockwood, Edward J.; Smith, Scott D.; Bonilha, Vera L.; Crabb, John S.; Kuchtey, Rachel W.; Robertson, Nahid G.; Peachey, Neal S.; Morton, Cynthia C.; Crabb, John W.

In: Journal of Biological Chemistry, Vol. 280, No. 7, 18.02.2005, p. 6080-6084.

Research output: Contribution to journalArticle

Bhattacharya, SK, Rockwood, EJ, Smith, SD, Bonilha, VL, Crabb, JS, Kuchtey, RW, Robertson, NG, Peachey, NS, Morton, CC & Crabb, JW 2005, 'Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork', Journal of Biological Chemistry, vol. 280, no. 7, pp. 6080-6084. https://doi.org/10.1074/jbc.M411233200
Bhattacharya, Sanjoy K ; Rockwood, Edward J. ; Smith, Scott D. ; Bonilha, Vera L. ; Crabb, John S. ; Kuchtey, Rachel W. ; Robertson, Nahid G. ; Peachey, Neal S. ; Morton, Cynthia C. ; Crabb, John W. / Proteomics reveal cochlin deposits associated with glaucomatous trabecular meshwork. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 7. pp. 6080-6084.
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