Proteomic analysis of the palmitate-induced myotube secretome reveals involvement of the annexin A1-formyl peptide receptor 2 (FPR2) pathway in insulin resistance

Jong Hyuk Yoon, Dayea Kim, Jin Hyeok Jang, Jaewang Ghim, Soyeon Park, Parkyong Song, Yonghoon Kwon, Jaeyoon Kim, Daehee Hwang, Yoe Sik Bae, Pann Ghill Suh, Per Olof Berggren, Sung Ho Ryu

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Elevated levels of the free fatty acid palmitate are found in the plasma of obese patients and induce insulin resistance. Skeletal muscle secretes myokines as extracellular signaling mediators in response to pathophysiological conditions. Here, we identified and characterized the skeletal muscle secretome in response to palmitate-induced insulin resistance. Using a quantitative proteomic approach, we identified 36 secretory proteins modulated by palmitate-induced insulin resistance. Bioinformatics analysis revealed that palmitate-induced insulin resistance induced cellular stress and modulated secretory events. We found that the decrease in the level of annexin A1, a secretory protein, depended on palmitate, and that annexin A1 and its receptor, formyl peptide receptor 2 agonist, played a protective role in the palmitate-induced insulin resistance of L6 myotubes through PKC-θ modulation. In mice fed with a high-fat diet, treatment with the formyl peptide receptor 2 agonist improved systemic insulin sensitivity. Thus, we identified myokine candidates modulated by palmitate-induced insulin resistance and found that the annexin A1-formyl peptide receptor 2 pathway mediated the insulin resistance of skeletal muscle, as well as systemic insulin sensitivity.

Original languageEnglish (US)
Pages (from-to)882-892
Number of pages11
JournalMolecular and Cellular Proteomics
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

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