Abstract
Proteomic analyses of zebra finch (Taeniopygia guttata) optic tectum resulted in identification of 176 proteins. In the Swissprot database, only 52 proteins were identified as bird homologs and only 71 proteins were identified in songbird transcriptome databases, reflecting a lack of completeness in the T. guttata genomic sequence. Analysis in Kyoto encyclopedia of genes and genome (KEGG) pathway database found that identified proteins most frequently belong to glucose, pyruvate, glyoxylate, dicarboxylate, alanine, and aspartate metabolism pathways. A number of identified proteins have been previously reported to exist in the avian optic tectum. The immunohistochemical localization of selected proteins showed their distribution in similar laminae of the owl (Tyto alba) and chicken (Gallus gallus) tectum. Immunohistochemical analysis of identified proteins can provide clues about cell types and circuit layout of the avian optic tectum in general. As the optic tectum of nonmammals is homologous to the superior colliculus of mammals, the analysis of the tectal and collicular proteome may provide clues about conserved cell and circuit layout, circuit function, and evolution.
| Original language | English |
|---|---|
| Pages (from-to) | 2341-2350 |
| Number of pages | 10 |
| Journal | Journal of Proteome Research |
| Volume | 6 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2007 |
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Keywords
- Circuit function
- Optic tectum
- Proteome
- Taeniopygia guttata
- Zebra finch
ASJC Scopus subject areas
- Genetics
- Biotechnology
- Biochemistry
Cite this
Proteomic analyses of zebra finch optic tectum and comparative histochemistry. / Sloley, Stephanie; Smith, Shannon; Gandhi, Sonia; Caldwell Busby, Jennifer A.; London, Sarah; Luksch, Harald; Clayton, David F.; Bhattacharya, Sanjoy K.
In: Journal of Proteome Research, Vol. 6, No. 6, 01.06.2007, p. 2341-2350.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic analyses of zebra finch optic tectum and comparative histochemistry
AU - Sloley, Stephanie
AU - Smith, Shannon
AU - Gandhi, Sonia
AU - Caldwell Busby, Jennifer A.
AU - London, Sarah
AU - Luksch, Harald
AU - Clayton, David F.
AU - Bhattacharya, Sanjoy K
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Proteomic analyses of zebra finch (Taeniopygia guttata) optic tectum resulted in identification of 176 proteins. In the Swissprot database, only 52 proteins were identified as bird homologs and only 71 proteins were identified in songbird transcriptome databases, reflecting a lack of completeness in the T. guttata genomic sequence. Analysis in Kyoto encyclopedia of genes and genome (KEGG) pathway database found that identified proteins most frequently belong to glucose, pyruvate, glyoxylate, dicarboxylate, alanine, and aspartate metabolism pathways. A number of identified proteins have been previously reported to exist in the avian optic tectum. The immunohistochemical localization of selected proteins showed their distribution in similar laminae of the owl (Tyto alba) and chicken (Gallus gallus) tectum. Immunohistochemical analysis of identified proteins can provide clues about cell types and circuit layout of the avian optic tectum in general. As the optic tectum of nonmammals is homologous to the superior colliculus of mammals, the analysis of the tectal and collicular proteome may provide clues about conserved cell and circuit layout, circuit function, and evolution.
AB - Proteomic analyses of zebra finch (Taeniopygia guttata) optic tectum resulted in identification of 176 proteins. In the Swissprot database, only 52 proteins were identified as bird homologs and only 71 proteins were identified in songbird transcriptome databases, reflecting a lack of completeness in the T. guttata genomic sequence. Analysis in Kyoto encyclopedia of genes and genome (KEGG) pathway database found that identified proteins most frequently belong to glucose, pyruvate, glyoxylate, dicarboxylate, alanine, and aspartate metabolism pathways. A number of identified proteins have been previously reported to exist in the avian optic tectum. The immunohistochemical localization of selected proteins showed their distribution in similar laminae of the owl (Tyto alba) and chicken (Gallus gallus) tectum. Immunohistochemical analysis of identified proteins can provide clues about cell types and circuit layout of the avian optic tectum in general. As the optic tectum of nonmammals is homologous to the superior colliculus of mammals, the analysis of the tectal and collicular proteome may provide clues about conserved cell and circuit layout, circuit function, and evolution.
KW - Circuit function
KW - Optic tectum
KW - Proteome
KW - Taeniopygia guttata
KW - Zebra finch
UR - http://www.scopus.com/inward/record.url?scp=34250825382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250825382&partnerID=8YFLogxK
U2 - 10.1021/pr070126w
DO - 10.1021/pr070126w
M3 - Article
C2 - 17497909
AN - SCOPUS:34250825382
VL - 6
SP - 2341
EP - 2350
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 6
ER -