Proteomic analyses of zebra finch optic tectum and comparative histochemistry

Stephanie Sloley, Shannon Smith, Sonia Gandhi, Jennifer A. Caldwell Busby, Sarah London, Harald Luksch, David F. Clayton, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Proteomic analyses of zebra finch (Taeniopygia guttata) optic tectum resulted in identification of 176 proteins. In the Swissprot database, only 52 proteins were identified as bird homologs and only 71 proteins were identified in songbird transcriptome databases, reflecting a lack of completeness in the T. guttata genomic sequence. Analysis in Kyoto encyclopedia of genes and genome (KEGG) pathway database found that identified proteins most frequently belong to glucose, pyruvate, glyoxylate, dicarboxylate, alanine, and aspartate metabolism pathways. A number of identified proteins have been previously reported to exist in the avian optic tectum. The immunohistochemical localization of selected proteins showed their distribution in similar laminae of the owl (Tyto alba) and chicken (Gallus gallus) tectum. Immunohistochemical analysis of identified proteins can provide clues about cell types and circuit layout of the avian optic tectum in general. As the optic tectum of nonmammals is homologous to the superior colliculus of mammals, the analysis of the tectal and collicular proteome may provide clues about conserved cell and circuit layout, circuit function, and evolution.

Original languageEnglish (US)
Pages (from-to)2341-2350
Number of pages10
JournalJournal of Proteome Research
Volume6
Issue number6
DOIs
StatePublished - Jun 1 2007

Keywords

  • Circuit function
  • Optic tectum
  • Proteome
  • Taeniopygia guttata
  • Zebra finch

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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  • Cite this

    Sloley, S., Smith, S., Gandhi, S., Caldwell Busby, J. A., London, S., Luksch, H., Clayton, D. F., & Bhattacharya, S. K. (2007). Proteomic analyses of zebra finch optic tectum and comparative histochemistry. Journal of Proteome Research, 6(6), 2341-2350. https://doi.org/10.1021/pr070126w