Proteomic analyses of songbird (Zebra finch; Taeniopygia guttata) retina

Stephanie Sloley, Shannon Smith, Mabel Algeciras, Valerie Cavett, Jennifer A. Caldwell Busby, Sarah London, David F. Clayton, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Proteomic analyses of male songbird (Zebra finch; Taeniopygia guttata; ZF) retina were performed resulting in identification of 129 proteins. Comparison of T. guttata retinal proteome with that of chicken found proteins detected in both retinas. Immunohistochemical analyses of T. guttata retinal sections and Western analyses of total retinal protein extract were performed confirming presence of select bona fide retinal proteins. Results demonstrate the utility of one-dimensional gel fractionation for mass spectrometry and will be useful for future proteomic comparison of songbird retina and brain tissues in different behavioral and pharmacological studies.

Original languageEnglish (US)
Pages (from-to)1093-1100
Number of pages8
JournalJournal of Proteome Research
Volume6
Issue number3
DOIs
StatePublished - Mar 1 2007

Keywords

  • 1D SDS-PAGE
  • Proteome
  • Retina
  • Songbird
  • Zebra finch

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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    Sloley, S., Smith, S., Algeciras, M., Cavett, V., Caldwell Busby, J. A., London, S., Clayton, D. F., & Bhattacharya, S. K. (2007). Proteomic analyses of songbird (Zebra finch; Taeniopygia guttata) retina. Journal of Proteome Research, 6(3), 1093-1100. https://doi.org/10.1021/pr060428i