Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282

Jawed Shafqat; Moin Ishrat; Theres Jägerbrink; Rannar Sillard; Uno Mäeorg; Suad Efendic; Per Olof Berggren; Sergei V. Zaitsev; Hans Jörnvall

doi:10.1016/j.febslet.2008.04.007

Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282

Jawed Shafqat, Moin Ishrat, Theres Jägerbrink, Rannar Sillard, Uno Mäeorg, Suad Efendic, Per Olof Berggren, Sergei V. Zaitsev, Hans Jörnvall

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Abstract

The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.

Original language	English (US)
Pages (from-to)	1613-1617
Number of pages	5
Journal	FEBS letters
Volume	582
Issue number	11
DOIs	https://doi.org/10.1016/j.febslet.2008.04.007
State	Published - May 14 2008

Keywords

Elongation factor 1A
Glucose regulated protein 94
Imidazoline compounds
MIN6 cells
Surface plasmon resonance

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282. / Shafqat, Jawed; Ishrat, Moin; Jägerbrink, Theres; Sillard, Rannar; Mäeorg, Uno; Efendic, Suad; Berggren, Per Olof; Zaitsev, Sergei V.; Jörnvall, Hans.

In: FEBS letters, Vol. 582, No. 11, 14.05.2008, p. 1613-1617.

Research output: Contribution to journal › Article › peer-review

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title = "Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282",

abstract = "The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.",

keywords = "Elongation factor 1A, Glucose regulated protein 94, Imidazoline compounds, MIN6 cells, Surface plasmon resonance",

author = "Jawed Shafqat and Moin Ishrat and Theres J{\"a}gerbrink and Rannar Sillard and Uno M{\"a}eorg and Suad Efendic and Berggren, {Per Olof} and Zaitsev, {Sergei V.} and Hans J{\"o}rnvall",

note = "Funding Information: We are grateful to Carina Palmberg and Gunvor Alvelius for skilful assistance. This work was supported by funds from the Swedish Research Council (Project 03X-3532), Karolinska Institutet, the Novo-Nordisk Foundation, the Swedish Diabetes Association, Knut and Alice Wallenberg Foundation, The Family Erling-Persson Foundation, and Berth von Kantzow{\textquoteright}s Foundation. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.febslet.2008.04.007",

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AU - Ishrat, Moin

AU - Jägerbrink, Theres

AU - Sillard, Rannar

AU - Mäeorg, Uno

AU - Efendic, Suad

AU - Berggren, Per Olof

AU - Zaitsev, Sergei V.

AU - Jörnvall, Hans

N1 - Funding Information: We are grateful to Carina Palmberg and Gunvor Alvelius for skilful assistance. This work was supported by funds from the Swedish Research Council (Project 03X-3532), Karolinska Institutet, the Novo-Nordisk Foundation, the Swedish Diabetes Association, Knut and Alice Wallenberg Foundation, The Family Erling-Persson Foundation, and Berth von Kantzow’s Foundation. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2008/5/14

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N2 - The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.

AB - The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.

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KW - Surface plasmon resonance

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Proteins in the insulin-secreting cell line MIN6 bind the imidazoline compound BL11282

Abstract

Keywords

ASJC Scopus subject areas

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