Abstract
The imidazoline BL11282 stimulates insulin release and alters islet proteomes. Subcellular fractions of MIN6 cells showed that the membrane fraction exhibited binding to BL11282 on a Biacore chip and to BL11282-labelled magnetic beads. Bound material extracted from the beads showed a ∼50 kDa differential band upon SDS-PAGE and a weaker 100 kDa band. The former was sensitive to competitive removal by preincubation of the fraction with BL11282, then highlighting the ∼100 kDa band. Masspectrometric analysis revealed the ∼50 kDa band to be EF1A and the ∼100 kDa band to be glucose regulated P94, both of interest in insulin synthesis and secretion.
Original language | English (US) |
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Pages (from-to) | 1613-1617 |
Number of pages | 5 |
Journal | FEBS letters |
Volume | 582 |
Issue number | 11 |
DOIs | |
State | Published - May 14 2008 |
Keywords
- Elongation factor 1A
- Glucose regulated protein 94
- Imidazoline compounds
- MIN6 cells
- Surface plasmon resonance
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology