Protein–lipid complex separation utilizing a capillary electrophoresis system

Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The separation and analysis of protein–lipid complexes has proven to be challenging due to the harsh conditions required by conventional methods of protein or lipid isolation, which disrupt the fine forces that govern the interactions between lipid head groups and protein side chains. The method described in this publication presents an alternative for the separation of protein–lipid complexes while maintaining the integrity of their interactions. The method exploits the specific electrophoretic forces that are unique to the geometry of the capillary system and allows purification of intact complexes and the systematic analysis of its constituents. This technique is specifically applied for the separation of native protein-lipid complexes found in the central nervous system.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-100
Number of pages6
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Capillary electrophoresis
  • Central nervous systems
  • Chromatography
  • Fused silica capillary
  • Protein–lipid interaction
  • Protein–lipid separation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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