Protein–lipid complex separation utilizing a capillary electrophoresis system

Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The separation and analysis of protein–lipid complexes has proven to be challenging due to the harsh conditions required by conventional methods of protein or lipid isolation, which disrupt the fine forces that govern the interactions between lipid head groups and protein side chains. The method described in this publication presents an alternative for the separation of protein–lipid complexes while maintaining the integrity of their interactions. The method exploits the specific electrophoretic forces that are unique to the geometry of the capillary system and allows purification of intact complexes and the systematic analysis of its constituents. This technique is specifically applied for the separation of native protein-lipid complexes found in the central nervous system.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-100
Number of pages6
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745

Fingerprint

Capillary Electrophoresis
Lipids
Proteins
Central Nervous System

Keywords

  • Capillary electrophoresis
  • Central nervous systems
  • Chromatography
  • Fused silica capillary
  • Protein–lipid interaction
  • Protein–lipid separation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Valdivia, A. O., Myer, C., Suarez, M. F., & Bhattacharya, S. K. (2019). Protein–lipid complex separation utilizing a capillary electrophoresis system. In Methods in Molecular Biology (pp. 95-100). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_9

Protein–lipid complex separation utilizing a capillary electrophoresis system. / Valdivia, Anddre Osmar; Myer, Ciara; Suarez, Maria Fernanda; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 95-100 (Methods in Molecular Biology; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceedingChapter

Valdivia, AO, Myer, C, Suarez, MF & Bhattacharya, SK 2019, Protein–lipid complex separation utilizing a capillary electrophoresis system. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1996, Humana Press Inc., pp. 95-100. https://doi.org/10.1007/978-1-4939-9488-5_9
Valdivia AO, Myer C, Suarez MF, Bhattacharya SK. Protein–lipid complex separation utilizing a capillary electrophoresis system. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 95-100. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9488-5_9
Valdivia, Anddre Osmar ; Myer, Ciara ; Suarez, Maria Fernanda ; Bhattacharya, Sanjoy K. / Protein–lipid complex separation utilizing a capillary electrophoresis system. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 95-100 (Methods in Molecular Biology).
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