Protein synthesis and rapid axonal transport during regrowth of dorsal root axons

G. W. Perry, S. R. Krayanek, D. L. Wilson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Damage to the sciatic nerve produces significant changes in the relative synthesis rates of some proteins in dorsal root ganglia and in the amounts of some fast axonally transported proteins in both the sicatic nerve and dorsal roots. We have now analyzed protein synthesis and axonal transport after cutting the other branch of dorsal root ganglia neurons, the dorsal roots. Two to three weeks after cutting the dorsal roots, [35S]methionine was used to label proteins in the dorsal root ganglia in vitro. Proteins synthesized in the dorsal root ganglia and transported along the sciatic nerve were analyzed on two-dimensional gels. All of the proteins previously observed to change after sciatic nerve damage were included in this study. No significant changes in proteins synthesized in dorsal root ganglia or rapidly transported along the sciatic nerve were detected. Axon regrowth from cut dorsal roots was observed by light and electron microscopy. Either the response to dorsal root damage is too small to be detected by our methods or changes in protein synthesis and fast axonal transports are not necessary for axon regrowth. When such changes do occur they may still aid in regrowth or be necessary for later stages in regeneration.

Original languageEnglish
Pages (from-to)1590-1598
Number of pages9
JournalJournal of Neurochemistry
Volume40
Issue number6
StatePublished - Jan 1 1983

Fingerprint

Axonal Transport
Spinal Nerve Roots
Axons
Spinal Ganglia
Sciatic Nerve
Proteins
Protein Transport
Methionine
Regeneration
Electron Microscopy
Electron microscopy
Neurons
Optical microscopy
Labels
Gels
Light

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Perry, G. W., Krayanek, S. R., & Wilson, D. L. (1983). Protein synthesis and rapid axonal transport during regrowth of dorsal root axons. Journal of Neurochemistry, 40(6), 1590-1598.

Protein synthesis and rapid axonal transport during regrowth of dorsal root axons. / Perry, G. W.; Krayanek, S. R.; Wilson, D. L.

In: Journal of Neurochemistry, Vol. 40, No. 6, 01.01.1983, p. 1590-1598.

Research output: Contribution to journalArticle

Perry, GW, Krayanek, SR & Wilson, DL 1983, 'Protein synthesis and rapid axonal transport during regrowth of dorsal root axons', Journal of Neurochemistry, vol. 40, no. 6, pp. 1590-1598.
Perry, G. W. ; Krayanek, S. R. ; Wilson, D. L. / Protein synthesis and rapid axonal transport during regrowth of dorsal root axons. In: Journal of Neurochemistry. 1983 ; Vol. 40, No. 6. pp. 1590-1598.
@article{18918a2364814276b6b32bf4e4a0609c,
title = "Protein synthesis and rapid axonal transport during regrowth of dorsal root axons",
abstract = "Damage to the sciatic nerve produces significant changes in the relative synthesis rates of some proteins in dorsal root ganglia and in the amounts of some fast axonally transported proteins in both the sicatic nerve and dorsal roots. We have now analyzed protein synthesis and axonal transport after cutting the other branch of dorsal root ganglia neurons, the dorsal roots. Two to three weeks after cutting the dorsal roots, [35S]methionine was used to label proteins in the dorsal root ganglia in vitro. Proteins synthesized in the dorsal root ganglia and transported along the sciatic nerve were analyzed on two-dimensional gels. All of the proteins previously observed to change after sciatic nerve damage were included in this study. No significant changes in proteins synthesized in dorsal root ganglia or rapidly transported along the sciatic nerve were detected. Axon regrowth from cut dorsal roots was observed by light and electron microscopy. Either the response to dorsal root damage is too small to be detected by our methods or changes in protein synthesis and fast axonal transports are not necessary for axon regrowth. When such changes do occur they may still aid in regrowth or be necessary for later stages in regeneration.",
author = "Perry, {G. W.} and Krayanek, {S. R.} and Wilson, {D. L.}",
year = "1983",
month = "1",
day = "1",
language = "English",
volume = "40",
pages = "1590--1598",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Protein synthesis and rapid axonal transport during regrowth of dorsal root axons

AU - Perry, G. W.

AU - Krayanek, S. R.

AU - Wilson, D. L.

PY - 1983/1/1

Y1 - 1983/1/1

N2 - Damage to the sciatic nerve produces significant changes in the relative synthesis rates of some proteins in dorsal root ganglia and in the amounts of some fast axonally transported proteins in both the sicatic nerve and dorsal roots. We have now analyzed protein synthesis and axonal transport after cutting the other branch of dorsal root ganglia neurons, the dorsal roots. Two to three weeks after cutting the dorsal roots, [35S]methionine was used to label proteins in the dorsal root ganglia in vitro. Proteins synthesized in the dorsal root ganglia and transported along the sciatic nerve were analyzed on two-dimensional gels. All of the proteins previously observed to change after sciatic nerve damage were included in this study. No significant changes in proteins synthesized in dorsal root ganglia or rapidly transported along the sciatic nerve were detected. Axon regrowth from cut dorsal roots was observed by light and electron microscopy. Either the response to dorsal root damage is too small to be detected by our methods or changes in protein synthesis and fast axonal transports are not necessary for axon regrowth. When such changes do occur they may still aid in regrowth or be necessary for later stages in regeneration.

AB - Damage to the sciatic nerve produces significant changes in the relative synthesis rates of some proteins in dorsal root ganglia and in the amounts of some fast axonally transported proteins in both the sicatic nerve and dorsal roots. We have now analyzed protein synthesis and axonal transport after cutting the other branch of dorsal root ganglia neurons, the dorsal roots. Two to three weeks after cutting the dorsal roots, [35S]methionine was used to label proteins in the dorsal root ganglia in vitro. Proteins synthesized in the dorsal root ganglia and transported along the sciatic nerve were analyzed on two-dimensional gels. All of the proteins previously observed to change after sciatic nerve damage were included in this study. No significant changes in proteins synthesized in dorsal root ganglia or rapidly transported along the sciatic nerve were detected. Axon regrowth from cut dorsal roots was observed by light and electron microscopy. Either the response to dorsal root damage is too small to be detected by our methods or changes in protein synthesis and fast axonal transports are not necessary for axon regrowth. When such changes do occur they may still aid in regrowth or be necessary for later stages in regeneration.

UR - http://www.scopus.com/inward/record.url?scp=0020541926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020541926&partnerID=8YFLogxK

M3 - Article

VL - 40

SP - 1590

EP - 1598

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 6

ER -